[Inhibitory Activity of Flower Extracts from Schang on ].

OBJECTIVE

To examine the inhibitory effect of flower extracts from Schang (SFE) on ( ).

METHODS

The inhibitory effect of SFE on planktonic and the effect of SFE on the growth process of planktonic were determined by the agar drilling method and the microdilution method. Crystal violet staining and MTT reduction assay were conducted to determine the effect of SFE on biofilm formation. The effect of SFE on the production of exopolysaccharides (EPS) in biofilm was determined by anthrone-sulfuric acid method. The intracellular lactate dehydrogenase (LDH) activity in was determined by LDH colorimetric assay. The effects of SFE on the acid-producing capacity of was determined by pH meter.

RESULTS

The minimum inhibitory concentration (MIC) of SFE against was 14 μg/μL. SFE of the the concentration between 1/8 MIC and MIC could inhibit the growth rate of within 30 h and it could significantly inhibit the LDH activity compared with the control group ( <0.0001). SFE of the concentration between 4 MIC and 1/4 MIC had an inhibitory effect on the acid production of ( <0.001). Moreover, it could effectively restrain the formation of biofilm and significantly reduce the amount of EPS produced by biofilm ( <0.01).

CONCLUSION

SFE can effectively inhibit the activity of and its biofilm. The mechanism of inhibiting by SFE was preliminarily discussed as follows, it interferes with microbial adhesion and aggregation by reducing the production of bacterial EPS, thus inhibiting the formation of bacterial biofilms. In addition, it interferes with glycolysis of by reducing the LDH activity of bacteria, thus inhibiting the acid production of .