Department of Food Science and Technology and Foodborne Virus Research Center, Chonnam National University, Gwangju 61186, Republic of Korea.
Department of BioChemical Analysis, Korea Basic Science Institute, Daejeon 34133, Republic of Korea.
J Food Prot. 2022 Jun 1;85(6):924-929. doi: 10.4315/JFP-21-419.
The challenges associated with development of an animal model system to replicate human norovirus (HuNoV) has hampered the study of the pathogenesis and therapeutic interventions for this virus. In this study, we replicated HuNoV GII.4 and evaluated virus gene expression in infected zebrafish. Three doses of inoculation resulted in successful virus replication. Genes for transmembrane transporters (tfa, cftr, slc26a3, and slc26a6), a heat shock chaperone (hspa8), and immune response cytokines (ifng1 and il1b) were highly expressed in HuNoV-infected zebrafish; however, expression levels of genes were reduced in zebrafish infected with thermally inactivated HuNoV. These results confirm HuNoV replication in juvenile zebrafish and will facilitate the investigation of biomarker gene expression during HuNoV infection.
开发能够复制人类诺如病毒 (HuNoV) 的动物模型系统所面临的挑战，阻碍了对该病毒发病机制和治疗干预措施的研究。在这项研究中，我们复制了 HuNoV GII.4 并评估了感染斑马鱼中的病毒基因表达。三次接种剂量导致成功的病毒复制。跨膜转运体 (tfa、cftr、slc26a3 和 slc26a6)、热休克伴侣蛋白 (hspa8) 和免疫反应细胞因子 (ifng1 和 il1b) 的基因在 HuNoV 感染的斑马鱼中高度表达；然而，在感染热失活 HuNoV 的斑马鱼中，基因的表达水平降低。这些结果证实了幼龄斑马鱼中 HuNoV 的复制，并将促进在 HuNoV 感染期间生物标志物基因表达的研究。
