Molecular Dermatology Laboratory, Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City 16419, Korea.
Department of Bio and Chemical Engineering, Hongik University, Sejong City 30016, Korea.
Nutrients. 2022 Mar 13;14(6):1217. doi: 10.3390/nu14061217.
While harmful effects of blue light on skin cells have been recently reported, there are few studies regarding natural products that alleviate its negative effects. Therefore, we investigated ameliorating effects of yellow chaste weed (YCW) () extract and its components, apigenin and galangin, on blue light-irradiated HaCaT cells. In this study, we found that YCW extract improved the reduced proliferation of HaCaT cells induced by blue light-irradiation and reduced blue light-induced production of reactive oxygen species (ROS) levels. We also found that apigenin and galangin, the main components of YCW extract, showed the same activities as YCW extract. In experiments examining molecular mechanisms of YCW extract and its components such as apigenin and galangin, they all reduced expression of transient receptor potential vanilloid member 1 (TRPV1), its phosphorylation, and calcium ion (Ca) influx induced by blue light irradiation. In addition, apigenin and galangin regulated phosphorylation of mitogen-activated protein kinases (MAPKs). They also reduced phosphorylation of mammalian sterile 20-like kinase-1/2 (MST-1/2), inducing phosphorylation of Akt (protein kinase B), one downstream molecule of MST-1/2. Moreover, apigenin and galangin promoted translocation of Forkhead box O3 (FoxO3a) from the nucleus to the cytosol by phosphorylating FoxO3a. Besides, apigenin and galangin interrupted blue light influences on expression of nuclear and secretory clusterin. Namely, they attenuated both upregulation of nuclear clusterin and downregulation of secretory clusterin induced by blue light irradiation. We also found that they downregulated apoptotic protein Bcl-2 associated X protein (Bax) and conversely upregulated anti-apoptotic protein B-cell lymphoma 2 (Bcl-2). Collectively, these findings indicate that YCW extract and its components, apigenin and galangin, antagonize the blue light-induced damage to the keratinocytes by regulating TRPV1/clusterin/FoxO3a and MAPK signaling.
虽然最近有报道称蓝光对皮肤细胞有有害影响，但关于能减轻其负面影响的天然产物的研究较少。因此，我们研究了黄荆（YCW）提取物及其成分芹菜素和高良姜素对受蓝光照射的 HaCaT 细胞的改善作用。在这项研究中，我们发现 YCW 提取物改善了蓝光照射诱导的 HaCaT 细胞增殖减少，并降低了蓝光诱导的活性氧（ROS）水平的产生。我们还发现，YCW 提取物的主要成分芹菜素和高良姜素表现出与 YCW 提取物相同的活性。在研究 YCW 提取物及其成分，如芹菜素和高良姜素的分子机制的实验中，它们都降低了瞬时受体电位香草酸亚型 1（TRPV1）的表达及其磷酸化和钙离子（Ca2+）内流，这些都是由蓝光照射诱导的。此外，芹菜素和高良姜素调节丝裂原活化蛋白激酶（MAPKs）的磷酸化。它们还降低了哺乳动物无丝分裂 20 样激酶 1/2（MST-1/2）的磷酸化，诱导 MST-1/2 的下游分子 Akt（蛋白激酶 B）的磷酸化。此外，芹菜素和高良姜素通过磷酸化 Forkhead box O3（FoxO3a）促进 FoxO3a 从细胞核向细胞质转位。此外，芹菜素和高良姜素通过阻断核和分泌型 clusterin 的表达来干扰蓝光的影响。也就是说，它们减弱了蓝光照射诱导的核 clusterin 上调和分泌型 clusterin 下调。我们还发现，它们下调了促凋亡蛋白 Bcl-2 相关 X 蛋白（Bax），并上调了抗凋亡蛋白 B 细胞淋巴瘤 2（Bcl-2）。综上所述，这些发现表明，YCW 提取物及其成分，芹菜素和高良姜素，通过调节 TRPV1/clusterin/FoxO3a 和 MAPK 信号通路拮抗蓝光对角质形成细胞的损伤。
