Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo CP 11800, Uruguay.
Laboratorio de Farmacognosia y Productos Naturales, Departamento de Química Orgánica, Facultad de Química, Universidad de la República, Montevideo CP 11800, Uruguay.
Molecules. 2022 Mar 21;27(6):2002. doi: 10.3390/molecules27062002.
Antimicrobial resistance is a growing concern in public health and current research shows an important role for bacterial biofilms in recurrent or chronic infections. New strategies, therefore, are necessary to overcome antimicrobial resistance, through the development of new therapies that could alter or inhibit biofilm formation. In this sense, antibiofilm natural products are very promising. In this work, a bioprospection of antimicrobial and antibiofilm extracts from Uruguayan soil bacteria and insect gut bacteria was carried out. Extracts from extracellular broths were tested for their ability to inhibit planktonic cell growth and biofilm formation. Genomic analysis of ILBB55 was carried out. All extracts were able to inhibit the growth of, at least, one microorganism and several extracts showed MICs lower than 500 µg mL against microorganisms of clinical relevance (, , and ). Among the extracts evaluated for biofilm inhibition only ILBB55, from , was able to inhibit, (99%) and (62%) biofilms. Genomic analysis of this strain showed gene clusters similar to other clusters that code for known antimicrobial compounds. Our study revealed that extracts from soil bacteria and insect gut bacteria, especially from ILBB55, could be potential candidates for drug discovery to treat infectious diseases and inhibit and biofilms.
抗微生物药物耐药性是公共卫生领域日益关注的问题，目前的研究表明，细菌生物膜在复发性或慢性感染中起着重要作用。因此，需要新的策略来克服抗微生物药物耐药性，通过开发新的疗法来改变或抑制生物膜的形成。在这方面，抗生物膜天然产物具有很大的应用前景。在这项工作中，对来自乌拉圭土壤细菌和昆虫肠道细菌的抗微生物和抗生物膜提取物进行了生物勘探。从胞外肉汤中提取的物质被测试其抑制浮游细胞生长和生物膜形成的能力。对 ILBB55 进行了基因组分析。所有提取物均能抑制至少一种微生物的生长，并且几种提取物对具有临床相关性的微生物（、、和）的 MIC 值低于 500 µg mL。在评估的抑制生物膜的提取物中，只有来自的 ILBB55 能够抑制、（99%）和（62%）生物膜。对该菌株的基因组分析表明，存在与其他编码已知抗菌化合物的基因簇相似的基因簇。我们的研究表明，土壤细菌和昆虫肠道细菌的提取物，尤其是来自的 ILBB55，可能是治疗传染病和抑制和生物膜的药物发现的潜在候选物。
