Carvalho Fábio M, Teixeira-Santos Rita, Mergulhão Filipe J M, Gomes Luciana C
LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Microorganisms. 2020 Dec 23;9(1):27. doi: 10.3390/microorganisms9010027.
Medical device-associated infections (MDAI) are a critical problem due to the increasing usage of medical devices in the aging population. The inhibition of biofilm formation through the use of probiotics has received attention from the medical field in the last years. However, this sparse knowledge has not been properly reviewed, so that successful strategies for biofilm management can be developed. This study aims to summarize the relevant literature about the effect of probiotics and their metabolites on biofilm formation in medical devices using a PRISMA-oriented (Preferred Reporting Items for Systematic reviews and Meta-Analyses) systematic search and meta-analysis. This approach revealed that the use of probiotics and their products is a promising strategy to hinder biofilm growth by a broad spectrum of pathogenic microorganisms. The meta-analysis showed a pooled effect estimate for the proportion of biofilm reduction of 70% for biosurfactants, 76% for cell-free supernatants (CFS), 77% for probiotic cells and 88% for exopolysaccharides (EPS). This review also highlights the need to properly analyze and report data, as well as the importance of standardizing the in vitro culture conditions to facilitate the comparison between studies. This is essential to increase the predictive value of the studies and translate their findings into clinical applications.
由于医疗设备在老年人群中的使用日益增加，与医疗设备相关的感染（MDAI）成为一个关键问题。近年来，通过使用益生菌来抑制生物膜形成受到了医学领域的关注。然而，这些稀少的知识尚未得到恰当的综述，因此无法制定出成功的生物膜管理策略。本研究旨在通过采用面向PRISMA（系统评价和Meta分析的首选报告项目）的系统检索和Meta分析，总结有关益生菌及其代谢产物对医疗设备中生物膜形成影响的相关文献。该方法表明，使用益生菌及其产品是一种有前景的策略，可阻碍多种致病微生物的生物膜生长。Meta分析显示，生物表面活性剂使生物膜减少比例的合并效应估计值为70%，无细胞上清液（CFS）为76%，益生菌细胞为77%，胞外多糖（EPS）为88%。本综述还强调了正确分析和报告数据的必要性，以及标准化体外培养条件以促进研究间比较的重要性。这对于提高研究的预测价值并将其结果转化为临床应用至关重要。
