Cano Irene, Parker Abigail, Ward Georgia M, Green Matthew, Ross Stuart, Bignell John, Daumich Caroline, Kerr Rose, Feist Stephen W, Batista Frederico M
Cefas Weymouth Laboratory, International Centre of Excellence for Aquatic Animal Health, Barrack Road, Weymouth DT4 8UB, UK.
Department of Life Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD, UK.
Pathogens. 2022 Mar 8;11(3):329. doi: 10.3390/pathogens11030329.
In the last decade, declines in the population of wild blue mussels in the Tamar estuary (United Kingdom) have been noted. In archived samples collected from 2013 to 2019, between 7% (in 2013) and 18% (in 2019) showed large granulocytoma and haemocytic infiltration in the interstitial tissue of the digestive gland. Four samples were selected for gene Nanopore sequencing. A consensus sequence of 1449 bp showed nucleotide similarities between 99.93-100% with published sequences of . In situ hybridisation (ISH) confirmed the presence of DNA within individual granulocytes of granulocytomas and also in prokaryotic-like inclusion bodies within the digestive epithelial cells. The design of diagnostic tests for surveillance of including more specific ISH probes and sequencing the genome of the isolates infecting mussels, will shed more light on the pathogenicity and spread of this pathogen.
在过去十年中，已注意到英国塔马河口野生蓝贻贝数量有所下降。在2013年至2019年收集的存档样本中，7%（2013年）至18%（2019年）的样本在消化腺间质组织中出现大颗粒细胞瘤和血细胞浸润。选取了四个样本进行基因纳米孔测序。一个1449 bp的共有序列与已发表序列的核苷酸相似度在99.93%至100%之间。原位杂交（ISH）证实颗粒细胞瘤的单个粒细胞内以及消化上皮细胞内的原核样包涵体内存在[病原体名称未给出]DNA。用于监测[病原体名称未给出]的诊断测试设计，包括更特异的ISH探针以及对感染贻贝的分离株进行基因组测序，将有助于更深入了解这种病原体的致病性和传播情况。
