Cui Hongxiao, Sun Yajun, Lin Hua, Zhao Yan, Zhao Xin
College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China.
Department of Animal Science, McGill University, Montreal, QC H9X 3V9, Canada.
Pathogens. 2022 Mar 11;11(3):339. doi: 10.3390/pathogens11030339.
is a common pathogen which can secrete outer membrane vesicles (OMVs). However, the effect of OMVs from Serovar Typhimurium ( Typhimurium) of poultry origin on cells of the chicken innate immune system is not well known. In this study, Typhimurium OMVs were first isolated from three different poultry strains of , CVCC542, SALA, and SALB. In order to investigate the effect of OMVs on the maturation of monocytes into macrophages, both bone marrow-derived (BMD) monocytes and macrophage cell line HD11 cells were used. OMVs promoted the formation of monocyte dendrites in both types of cells, enabled BMD cells to become larger, and stimulated expression of LPS-induced TNF-αfactor (LITAF), IL-6, and inducible nitric oxide synthase (iNOS) genes in HD11 cells. These results demonstrated the capability of OMVs to promote the development of chicken monocytes into macrophages and the maturation of macrophages. In order to study the effect of OMVs on the phagocytosis of macrophages, chicken spleen-derived monocytes and HD11 cells were used. Phagocytosis of FITC- and FITC-dextran by these two types of cells was enhanced after stimulation with OMVs. To determine which components in OMVs were responsible for the above observed results, OMVs were treated with proteinase K(PK) or polymyxin B (PMB). Both treatments reduced the phagocytosis of FITC- by HD11 cells and chicken spleen mononuclear cells and reduced the secretion of IL-1β, LITAF, and IL-6 cytokines. These results demonstrated that OMVs activated chicken macrophages and spleen mononuclear cells and the activation was achieved mainly through lipopolysaccharides and membrane proteins.
是一种常见病原体，能够分泌外膜囊泡（OMV）。然而，源自家禽的鼠伤寒血清型（鼠伤寒）的OMV对鸡先天免疫系统细胞的影响尚不清楚。在本研究中，首先从三种不同的家禽菌株CVCC542、SALA和SALB中分离出鼠伤寒OMV。为了研究OMV对单核细胞成熟为巨噬细胞的影响，使用了骨髓来源（BMD）的单核细胞和巨噬细胞系HD11细胞。OMV促进了两种类型细胞中单核细胞树突的形成，使BMD细胞变大，并刺激HD11细胞中脂多糖诱导的肿瘤坏死因子-α因子（LITAF）、白细胞介素-6和诱导型一氧化氮合酶（iNOS）基因的表达。这些结果证明了OMV促进鸡单核细胞发育为巨噬细胞以及巨噬细胞成熟的能力。为了研究OMV对巨噬细胞吞噬作用的影响，使用了鸡脾脏来源的单核细胞和HD11细胞。用OMV刺激后，这两种类型细胞对异硫氰酸荧光素（FITC）和FITC-葡聚糖的吞噬作用增强。为了确定OMV中的哪些成分导致了上述观察结果，用蛋白酶K（PK）或多粘菌素B（PMB）处理OMV。两种处理均降低了HD11细胞和鸡脾脏单核细胞对FITC的吞噬作用，并减少了白细胞介素-1β、LITAF和白细胞介素-6细胞因子的分泌。这些结果表明，鼠伤寒OMV激活了鸡巨噬细胞和脾脏单核细胞，并且这种激活主要通过脂多糖和膜蛋白实现。
