Gomes Vasco T M, Moreno Luisa Z, Silva Ana Paula S, Thakur Siddhartha, La Ragione Roberto M, Mather Alison E, Moreno Andrea M
Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo 05508-270, Brazil.
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
Pathogens. 2022 Mar 16;11(3):358. doi: 10.3390/pathogens11030358.
Salmonellosis is a zoonosis of major relevance to global public health. Here we present the assessment of contamination in pork and poultry meat sold at retail markets in São Paulo, Brazil. A total of 780 meat samples (386 poultry meat and 394 pork samples) were collected from 132 markets. From these, 57 samples (7.3%) were positive for isolation, including 32 (8.3%) poultry meat and 25 (6.3%) pork samples. isolates were further characterized for serotyping, antimicrobial resistance and genotyping by amplified fragment length polymorphism and pulsed field gel electrophoresis. Antimicrobial resistance analysis demonstrated two main profiles: pork isolates were more resistant to macrolides, β-lactams, tetracycline, phenicols, and fluoroquinolones, and poultry meat isolates presented higher resistance to fluoroquinolones, sulfonamides, tetracycline, and β-lactams. A total of 72.4% of poultry meat isolates were identified as Heidelberg, while most of pork isolates were Typhimurium (31.7%) and . Give (16.7%). Genotyping resulted in most clusters consisting exclusively of pork or poultry meat, no cross-contamination was detected, and a tendency to differentiate isolates according to their serotypes and markets of origin. High resistance rates to critically important antimicrobials reinforce the importance of controlling Salmonella contamination in meat production chains.
沙门氏菌病是一种对全球公共卫生具有重大意义的人畜共患病。在此，我们展示了对巴西圣保罗零售市场销售的猪肉和禽肉污染情况的评估。从132个市场共采集了780份肉类样本（386份禽肉样本和394份猪肉样本）。其中，57份样本（7.3%）分离培养呈阳性，包括32份（8.3%）禽肉样本和25份（6.3%）猪肉样本。对分离株进一步进行血清型鉴定、抗菌药物耐药性分析以及通过扩增片段长度多态性和脉冲场凝胶电泳进行基因分型。抗菌药物耐药性分析显示出两种主要模式：猪肉分离株对大环内酯类、β-内酰胺类、四环素类、酚类和氟喹诺酮类药物的耐药性更强，而禽肉分离株对氟喹诺酮类、磺胺类、四环素类和β-内酰胺类药物的耐药性更高。总共72.4%的禽肉分离株被鉴定为海德堡沙门氏菌，而大多数猪肉分离株为鼠伤寒沙门氏菌（31.7%）和[此处原文缺失部分内容]（16.7%）。基因分型结果显示，大多数聚类仅由猪肉或禽肉分离株组成，未检测到交叉污染，并且存在根据血清型和来源市场区分分离株的趋势。对极其重要的抗菌药物的高耐药率凸显了控制肉类生产链中沙门氏菌污染的重要性。
