Gonçalves Carlota, Silveira Leonor, Rodrigues João, Furtado Rosália, Ramos Sónia, Nunes Alexandra, Pista Ângela
National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal.
Department of Chemistry, Nova School of Science & Technology, Campus da Caparica, 2829-516 Caparica, Portugal.
Antibiotics (Basel). 2024 Oct 11;13(10):957. doi: 10.3390/antibiotics13100957.
Foodborne diseases are a serious public health concern, and food-producing animals are a major source of contamination. The present study analysed and spp. isolated from faecal samples of 100 fattening pigs and from 52 samples of pork meat. The results showed that the majority of the analysed meat samples were considered satisfactory in terms of microbiological quality (92.3% for and 94.2% for spp.). spp. was identified in 5.8% of the meat samples, whereas was detected in 89.5% of all samples (69.2% in meat and 100% in faecal samples). Furthermore, 1.9% of the faecal samples contained Shiga-toxin-producing and 3.9% contained enterotoxigenic . All sequenced isolates presented virulence genes for extraintestinal pathogenic . Moreover, 75.0% of isolates from meat and 71.8% from faeces samples showed antibiotic resistance, with 40.7% and 51.4%, respectively, being multidrug-resistant (MDR). The most prevalent resistances were to tetracycline, ampicillin, and sulfamethoxazole, and one isolate showed resistance to extended-spectrum β-lactamase. This study highlights the role of pigs as a potential source of human contamination and the importance of a One Health approach to ensure food safety and to promote public health.
食源性疾病是一个严重的公共卫生问题，而食用动物是主要的污染源。本研究分析了从100头育肥猪的粪便样本和52份猪肉样本中分离出的[具体菌种1]和[具体菌种2]。结果表明，就微生物质量而言，大多数分析的肉类样本被认为是令人满意的（[具体菌种1]为92.3%，[具体菌种2]为94.2%）。[具体菌种2]在5.8%的肉类样本中被鉴定出来，而[具体菌种1]在所有样本的89.5%中被检测到（肉类中为69.2%，粪便样本中为100%）。此外，1.9%的粪便样本含有产志贺毒素的[具体菌种3]，3.9%含有产肠毒素的[具体菌种4]。所有测序分离株都呈现出肠道外致病性[具体菌种5]的毒力基因。此外，来自肉类的[具体菌种6]分离株中有75.0%和来自粪便样本的71.8%表现出抗生素耐药性，其中分别有40.7%和51.4%为多重耐药（MDR）。最普遍的耐药性是对四环素、氨苄青霉素和磺胺甲恶唑，并且有一株[具体菌种7]分离株表现出对超广谱β-内酰胺酶的耐药性。本研究强调了猪作为人类污染源的潜在作用以及“同一健康”方法对于确保食品安全和促进公共卫生的重要性。
