El Khoury Sarah, Giovenazzo Pierre, Derome Nicolas
Department of Biology, Laval University, Québec, QC G1V 0A6, Canada.
Institut de Biologie Intégrative et des Systèmes (IBIS), Laval University, Québec, QC G1V 0A6, Canada.
Microorganisms. 2022 Feb 23;10(3):493. doi: 10.3390/microorganisms10030493.
Including probiotics in honeybee nutrition represents a promising solution for mitigating diseases, and recent evidence suggests that various microbes possess mechanisms that can bioremediate environmental pollutants. Thus, the use of probiotics capable of degrading pesticides used in modern agriculture would help to both reduce colony losses due to the exposure of foragers to these toxic molecules and improve honeybee health and wellbeing globally. We conducted in vitro experiments to isolate and identify probiotic candidates from bacterial isolates of the honeybee gut (i.e., endogenous strains) according to their ability to (i) grow in contact with three sublethal concentrations of the pesticide clothianidin (0.15, 1 and 10 ppb) and (ii) degrade clothianidin at 0.15 ppb. The isolated bacterial strains were indeed able to grow in contact with the three sublethal concentrations of clothianidin. Bacterial growth rate differed significantly depending on the probiotic candidate and the clothianidin concentration used. Clothianidin was degraded by seven endogenous honeybee gut bacteria, namely sp., two sp., sp., sp., sp. and sp., measured within 72 h under in vitro conditions. Our findings highlight that endogenous bacterial strains may constitute the base material from which to develop a promising probiotic strategy to mitigate the toxic effects of clothianidin exposure on honeybee colony health.
在蜜蜂营养中添加益生菌是减轻疾病的一种有前景的解决方案，最近的证据表明，各种微生物拥有可对环境污染物进行生物修复的机制。因此，使用能够降解现代农业中使用的杀虫剂的益生菌，将有助于减少因觅食者接触这些有毒分子而导致的蜂群损失，并在全球范围内改善蜜蜂的健康状况。我们进行了体外实验，根据从蜜蜂肠道细菌分离物（即内源菌株）中分离和鉴定益生菌候选物的能力，即它们（i）在与三种亚致死浓度的农药噻虫胺（0.15、1和10 ppb）接触时生长的能力，以及（ii）在0.15 ppb浓度下降解噻虫胺的能力。分离出的细菌菌株确实能够在与三种亚致死浓度的噻虫胺接触时生长。细菌生长速率因益生菌候选物和所用噻虫胺浓度的不同而有显著差异。在体外条件下，72小时内，七种蜜蜂肠道内源细菌，即 种、两种 种、 种、 种、 种和 种，能够降解噻虫胺。我们的研究结果突出表明，内源细菌菌株可能构成开发一种有前景的益生菌策略的基础材料，以减轻噻虫胺暴露对蜜蜂蜂群健康的毒性影响。
