Department of Life Sciences & Convergence Research Center for Insect Vectors, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea.
Department of Life Sciences & Convergence Research Center for Insect Vectors, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea.
Pestic Biochem Physiol. 2020 Mar;164:221-227. doi: 10.1016/j.pestbp.2020.02.005. Epub 2020 Mar 1.
τ-Fluvalinate (fluvalinate) is a highly selective pyrethroid insecticide compound used for controlling ectoparasitic mites that cause major damages in honey bee colonies. Although honey bees have resistance and low toxicity to this xenobiotic chemical, little is known about the effects of this chemical on sensory modulation and behaviors in honey bees. Here we addressed the effect on olfactory cognition at the behavioral, molecular, and neurophysiological levels. First, we found that topical application of fluvalinate to honeybee abdomen elicited somewhat severe toxicity to honey bees. Furthermore, honeybees treated with sublethal doses of fluvalinate showed a significant decrease in olfactory responses. At the molecular level, there was no change in gene expression levels of odorant receptor co-receptor (Orco), which is important for electrical conductivity induced by odorant binding in insects. Rather, small neuropeptide F (sNPF) signaling pathway was involved in olfactory fluctuation after treatment of fluvalinate. This indicates that olfactory deficits by abdominal contact of fluvalinate may stem from various internal molecular pathways in honey bees.
τ-氰戊菊酯（fluvalinate）是一种高选择性的拟除虫菊酯杀虫剂化合物，用于控制导致蜜蜂群体重大损害的外寄生虫螨。尽管蜜蜂对这种异生物质化学物质具有抗性和低毒性，但对于这种化学物质对蜜蜂感官调节和行为的影响知之甚少。在这里，我们在行为、分子和神经生理学水平上研究了它对嗅觉认知的影响。首先，我们发现τ-氰戊菊酯涂敷在蜜蜂腹部会对蜜蜂产生一定程度的严重毒性。此外，用亚致死剂量的氟戊菊酯处理的蜜蜂对嗅觉反应明显下降。在分子水平上，气味受体共受体（Orco）的基因表达水平没有变化，Orco 对于昆虫中气味结合诱导的电导率很重要。相反，在氟戊菊酯处理后，小神经肽 F（sNPF）信号通路参与了嗅觉波动。这表明腹部接触氟戊菊酯导致的嗅觉缺陷可能源于蜜蜂体内的各种内部分子途径。
