Khan Anisa Sarah, Pierneef Rian Ewald, Gonzalez-Escalona Narjol, Maguire Meghan, Li Cong, Tyson Gregory H, Ayers Sherry, Georges Karla, Abebe Woubit, Adesiyun Abiodun Adewale
School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine 999183, Trinidad and Tobago.
Agricultural Research Council-Biotechnology Platform, Onderstepoort, 100 Old Soutpan Road, Pretoria 0110, South Africa.
Microorganisms. 2022 Mar 6;10(3):570. doi: 10.3390/microorganisms10030570.
This cross-sectional study determined the serovars, antimicrobial resistance genes, and virulence factors of isolated from hatcheries, broiler farms, processing plants, and retail outlets in Trinidad and Tobago. in silico serotyping detected 23 different serovars where Kentucky 20.5% (30/146), Javiana 19.2% (28/146), Infantis 13.7% (20/146), and Albany 8.9% (13/146) were the predominant serovars. There was a 76.0% (111/146) agreement between serotyping results using traditional conventional methods and whole-genome sequencing (WGS) in in silico analysis. In silico identification of antimicrobial resistance genes conferring resistance to aminoglycosides, cephalosporins, peptides, sulfonamides, and antiseptics were detected. Multidrug resistance (MDR) was detected in 6.8% (10/146) of the isolates of which 100% originated from broiler farms. Overall, virulence factors associated with secretion systems and fimbrial adherence determinants accounted for 69.3% (3091/4463), and 29.2% (1302/4463) counts, respectively. Ten of 20 isolates of serovar Infantis (50.0%) showed MDR and contained the gene. This is the first molecular characterization of isolates detected along the entire broiler production continuum in the Caribbean region using WGS. The availability of these genomes will help future source tracking during epidemiological investigations associated with foodborne outbreaks in the region and worldwide.
这项横断面研究确定了从特立尼达和多巴哥的孵化场、肉鸡养殖场、加工厂及零售网点分离出的[具体研究对象]的血清型、抗菌药物耐药基因和毒力因子。计算机血清型分析检测到23种不同的血清型，其中肯塔基血清型占20.5%（30/146），贾维亚纳血清型占19.2%（28/146），婴儿血清型占13.7%（20/146），奥尔巴尼血清型占8.9%（13/146），这些是主要的血清型。在计算机分析中，使用传统常规方法的血清型分析结果与全基因组测序（WGS）之间的一致性为76.0%（111/146）。检测到了赋予对氨基糖苷类、头孢菌素类、肽类、磺胺类和防腐剂耐药性的抗菌药物耐药基因。在6.8%（10/146）的分离株中检测到多重耐药（MDR），其中100%源自肉鸡养殖场。总体而言，与分泌系统和菌毛黏附决定因素相关的毒力因子分别占69.3%（3091/4463）和29.2%（1302/4463）。婴儿血清型的20株分离株中有10株（50.0%）显示多重耐药并含有[具体基因]。这是加勒比地区首次使用全基因组测序对肉鸡生产全过程中检测到的[具体研究对象]分离株进行分子特征分析。这些基因组的可得性将有助于在该地区及全球与食源性疾病暴发相关的流行病学调查中进行未来的溯源追踪。
