Khan Anisa Sarah, Georges Karla, Rahaman Saed, Abebe Woubit, Adesiyun Abiodun Adewale
School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago.
Veterinary Public Health Unit, Ministry of Health, Port of Spain, Trinidad and Tobago.
Microorganisms. 2021 May 13;9(5):1048. doi: 10.3390/microorganisms9051048.
This cross-sectional study determined the prevalence, characteristics, and risk factors for contamination of chicken with at four operating broiler processing plants in Trinidad. Standard methods were used to isolate and characterize the isolates. The overall prevalence of at the four processing plants was 27.0% (107/396). The whole carcass enrichment (WCE) method yielded a statistically significantly ( = 0.0014) higher frequency of isolation (53.9%; 97/180) than the whole carcass rinse (35.0%; 63/180) and neck skin methods (42.2%; 38/90). enterica serotypes Enteritidis, Javiana, and Infantis were the predominant serotypes isolated accounting for 20.8%, 16.7% and 12.5%, respectively, of the serotyped isolates. Risk factors included the use of over 100 contract farmers (OR 4.4), pre-chiller (OR 2.3), addition of chlorine to chiller (OR 3.2), slaughtering sick broilers (OR 4.4), and flocks with >50% mortality. Multi-drug resistance was detected in 12.3% (14/114) of the isolates of . Resistance was high to kanamycin (85.7%) and doxycycline (74.6%) but low to amoxicillin-clavulanic acid (2.4%) and sulphamethoxazole-trimethoprim (0.8%). The occurrence of resistant in chickens processed at commercial broiler processing plants has implications for salmonellosis and therapeutic failure in consumers of improperly cooked contaminated chickens from these plants in the country.
这项横断面研究确定了特立尼达四个肉鸡加工厂鸡肉中沙门氏菌污染的流行情况、特征和风险因素。采用标准方法分离并鉴定沙门氏菌分离株。四个加工厂沙门氏菌的总体流行率为27.0%（107/396）。全胴体富集（WCE）方法的分离频率（53.9%；97/180）在统计学上显著高于全胴体冲洗法（35.0%；63/180）和颈部皮肤法（42.2%；38/90）（P = 0.0014）。肠炎沙门氏菌、贾维亚纳沙门氏菌和婴儿沙门氏菌是分离出的主要血清型，分别占分型分离株的20.8%、16.7%和12.5%。风险因素包括使用100多个合同养殖户（比值比4.4）、预冷器（比值比2.3）、向冷却器中添加氯（比值比3.2）、宰杀病鸡（比值比4.4）以及死亡率超过50%的鸡群。在12.3%（14/114）的沙门氏菌分离株中检测到多重耐药性。对卡那霉素（85.7%）和强力霉素（74.6%）的耐药性较高，但对阿莫西林-克拉维酸（2.4%）和磺胺甲恶唑-甲氧苄啶（0.8%）的耐药性较低。在商业肉鸡加工厂加工的鸡肉中出现耐药性沙门氏菌，对该国这些工厂加工的受污染鸡肉烹饪不当的消费者的沙门氏菌病和治疗失败具有影响。
