Sile Inga, Krizhanovska Valerija, Nakurte Ilva, Mezaka Ieva, Kalane Laura, Filipovs Jevgenijs, Vecvanags Alekss, Pugovics Osvalds, Grinberga Solveiga, Dambrova Maija, Kronberga Arta
Latvian Institute of Organic Synthesis, 21 Aizkraukles Street, LV-1006 Riga, Latvia.
Department of Applied Pharmacy, Riga Stradins University, 16 Dzirciema Street, LV-1007 Riga, Latvia.
Plants (Basel). 2022 Mar 18;11(6):819. doi: 10.3390/plants11060819.
L. is a medicinal plant that is known in traditional medicine for its anti-inflammatory, antibacterial, antiviral, and anticancer properties. This study evaluated the potential for commercial production of and compared the chemical composition and activity of 70% ethanol extracts and steam-distilled essential oils from wild-grown and cultivated collected in different harvesting periods. The main compounds identified in the 70% ethanol extracts were phenolic acids (chlorogenic and rosmarinic acids) and flavonoid O-glycosides. The essential oil varied in the three accessions in the range of 0.32-2.98 mL/kg of dry weight. The extracts possessed potent antioxidant and anti-inflammatory properties in LPS-treated bone-marrow-derived macrophages. The results of flow cytometry show that extracts from different vegetation periods reduced the conversion of macrophages to the proinflammatory phenotype M1. The chemical composition varied the most with the different harvesting periods, and the most suitable periods were the flowering and vegetative phases for the polyphenolic compounds and essential oils, respectively. can be successfully grown under organic farming conditions, and cultivation does not significantly affect the chemical composition and biological activity compared to wild-grown plants.
L.是一种药用植物，在传统医学中因其抗炎、抗菌、抗病毒和抗癌特性而闻名。本研究评估了其商业生产潜力，并比较了不同采收期采集的野生和栽培L.的70%乙醇提取物和水蒸气蒸馏精油的化学成分及活性。70%乙醇提取物中鉴定出的主要化合物为酚酸（绿原酸和迷迭香酸）和黄酮O-糖苷。精油在三个种质中的含量在0.32-2.98 mL/kg干重范围内。提取物在脂多糖处理的骨髓来源巨噬细胞中具有强大的抗氧化和抗炎特性。流式细胞术结果表明，不同生长时期的提取物减少了巨噬细胞向促炎表型M1的转化。化学成分随采收期变化最大，多酚化合物和精油最适宜的采收期分别为花期和营养期。L.可以在有机种植条件下成功种植，与野生植物相比，种植对其化学成分和生物活性没有显著影响。
