Loarce Yolanda, Cabeza Alejandra, Cañas Rodrigo, González Juan M
Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, 28805 Alcalá de Henares, Spain.
Plants (Basel). 2022 Mar 19;11(6):821. doi: 10.3390/plants11060821.
Durum wheat ( 2n = 4x = AABB) includes several subspecies with differential characteristics in their root system architecture (RSA). Subspecies has longer and more vertical roots, while subspecies has smaller and shallower roots. The homeologous genes and of both subspecies have been identified and found to differ in their sizes, sequences and the proteins they encode. To determine whether there is a relationship between the level of expression of these two genes and the angle adopted by the roots of durum wheat seedlings, their expressions has been studied by RT-qPCR, both in the primary seminal root and in the other seminal roots. The results of the analyses showed that the gene is expressed 1.4 times more in the primary seminal root than in the other seminal roots. Furthermore, this gene is expressed 2.49 to 8.76 times more than depending on root type (primary or seminal) and subspecies. There are positive correlations between the expression ratio of both genes (/) and the mean of all root angles, the most vertical root angle and the most horizontal root angle of the seedlings. The higher the expression of gene, the lower the root growth angles.
硬粒小麦（2n = 4x = AABB）包括几个在根系结构（RSA）上具有不同特征的亚种。[此处原文中未提及具体亚种名称]亚种具有更长且更垂直的根，而[此处原文中未提及具体亚种名称]亚种的根更小且更浅。已鉴定出这两个亚种的同源基因[此处原文中未提及具体基因名称]和[此处原文中未提及具体基因名称]，发现它们在大小、序列以及所编码的蛋白质方面存在差异。为了确定这两个基因的表达水平与硬粒小麦幼苗根所采用的角度之间是否存在关系，通过RT-qPCR研究了它们在初生胚根和其他胚根中的表达情况。分析结果表明，[此处原文中未提及具体基因名称]基因在初生胚根中的表达量比在其他胚根中高1.4倍。此外，根据根的类型（初生或胚根）和亚种的不同，该基因的表达量比[此处原文中未提及具体基因名称]高2.49至8.76倍。两个基因的表达比率（[此处原文中未提及具体基因名称]/[此处原文中未提及具体基因名称]）与幼苗所有根角度的平均值、最垂直根角度和最水平根角度之间存在正相关。[此处原文中未提及具体基因名称]基因的表达越高，根的生长角度越低。
