Effects of Four Cropping Patterns of on Rhizosphere Microbiome Structure and Replant Disease.

Replant disease caused by continuous cropping obstacles commonly occurs in a consecutive monoculture. To reveal the mechanisms contributing to the continuous cropping obstacles of , four cropping patterns (fallow, -rice rotation, newly planted , and 2-year consecutive monoculture) were designed, and Illumina MiSeq (16S rDNA and ITS) was utilized to detect shifts in the microbial community in the rhizosphere. Our result showed that planting of significantly reduced soil pH. Consecutive monoculture of can significantly decrease the diversity and abundance of soil bacteria, but markedly increase the diversity and abundance of soil fungi. Under the four planting pattern treatments, the changes in soil pH were consistent with the changes in the Shannon diversity index of soil bacterial communities, whereas we observed a negative correlation between soil pH and Shannon diversity index for fungi. The relative abundance of Lactobacillales significantly increased in soils of consecutive monoculture, while Acidobacteriales, Solibacterales, and Xanthomonadales increased in soils of -rice rotation and newly planted . Collectively, this work aimed to elucidate the relationship between the planting patterns and soil microbiome, thereby providing a theoretical basis for screening new biological agents that may contribute to resolving continuous cropping obstacles of