Ma Wenyue, Liao Xiaolan, Wang Chong, Zhang Ya
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Plants (Basel). 2022 Mar 20;11(6):824. doi: 10.3390/plants11060824.
Replant disease caused by continuous cropping obstacles commonly occurs in a consecutive monoculture. To reveal the mechanisms contributing to the continuous cropping obstacles of , four cropping patterns (fallow, -rice rotation, newly planted , and 2-year consecutive monoculture) were designed, and Illumina MiSeq (16S rDNA and ITS) was utilized to detect shifts in the microbial community in the rhizosphere. Our result showed that planting of significantly reduced soil pH. Consecutive monoculture of can significantly decrease the diversity and abundance of soil bacteria, but markedly increase the diversity and abundance of soil fungi. Under the four planting pattern treatments, the changes in soil pH were consistent with the changes in the Shannon diversity index of soil bacterial communities, whereas we observed a negative correlation between soil pH and Shannon diversity index for fungi. The relative abundance of Lactobacillales significantly increased in soils of consecutive monoculture, while Acidobacteriales, Solibacterales, and Xanthomonadales increased in soils of -rice rotation and newly planted . Collectively, this work aimed to elucidate the relationship between the planting patterns and soil microbiome, thereby providing a theoretical basis for screening new biological agents that may contribute to resolving continuous cropping obstacles of
由连作障碍引起的再植病通常发生在连续单作中。为了揭示导致[作物名称]连作障碍的机制，设计了四种种植模式（休耕、[作物名称]-水稻轮作、新种植[作物名称]和两年连续单作），并利用Illumina MiSeq（16S rDNA和ITS）检测根际微生物群落的变化。我们的结果表明，种植[作物名称]显著降低了土壤pH值。[作物名称]的连续单作可显著降低土壤细菌的多样性和丰度，但显著增加土壤真菌的多样性和丰度。在四种种植模式处理下，土壤pH值的变化与土壤细菌群落的香农多样性指数变化一致，而我们观察到土壤pH值与真菌的香农多样性指数之间呈负相关。在[作物名称]连续单作的土壤中，乳杆菌目相对丰度显著增加，而在[作物名称]-水稻轮作和新种植[作物名称]的土壤中，酸杆菌目、厌氧绳菌目和黄单胞菌目增加。总的来说，这项工作旨在阐明[作物名称]种植模式与土壤微生物组之间的关系，从而为筛选可能有助于解决[作物名称]连作障碍的新型生物制剂提供理论依据。
