一种获取板层供体移植物的可选手术技术：初步研究。

An optional surgical technique for obtaining lamellar donor grafts: a pilot study.

机构信息

Department of Ophthalmology, Tongji Hospital, School of Medicine, Tongji University, No. 389, Xincun Road, Putuo District, Shanghai, 200092, China.

Department of Ophthalmology, Guizhou Provincial People's Hospital, Guiyang, Guizhou, China.

出版信息

BMC Ophthalmol. 2022 Mar 25;22(1):137. doi: 10.1186/s12886-022-02371-5.

DOI:10.1186/s12886-022-02371-5

PMID:35337290

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8957127/

Abstract

BACKGROUND

To evaluate the surface quality and thickness uniformity of lamellar donor grafts using an optional surgical technique called reversed manual dissection (RMD) in porcine corneas.

METHODS

Twenty-four paired porcine corneas (48 eyes) were numbered 1 to 24 and divided into 6 groups. All left corneas were assigned to conventional manual dissection (CMD), and all right corneas were assigned to RMD. Each group contained 8 corneas. For Groups I, II, and III, 30, 50, and 70% of the entire corneal thickness was dissected using CMD. For groups IV, V, and VI, 70, 50, and 30% of the entire corneal thickness was dissected using RMD. The residual stromal thickness was examined by anterior segment optical coherence tomography (ASOCT) to assess the thickness uniformity and scanning electron microscopy (SEM) to assess the surface quality.

RESULTS

The thickness uniformity of the lamellar grafts between each paired group was not significantly different (p > 0.05). The qualitative surface roughness grading (QiSR) evaluated by masked observers through SEM was significantly higher in the RMD groups (p < 0.001). The quantitative surface roughness grading (QnSR) acquired from the Mountains software was significantly lower in the RMD groups (p < 0.001).

CONCLUSIONS

RMD is an optional surgical technique for obtaining porcine lamellar grafts. The thickness uniformity of RMD is comparable to that of CMD, and a smoother surface with fewer ridges and roughness is achieved compared to CMD.

摘要

背景

评估使用一种名为反向手动解剖（RMD）的可选手术技术在猪角膜上获得的板层供体移植物的表面质量和厚度均匀性。

方法

将 24 对猪角膜（48 只眼）编号为 1 至 24 并分为 6 组。所有左眼角膜均被分配用于常规手动解剖（CMD），所有右眼角膜均被分配用于 RMD。每组包含 8 个角膜。对于第 I、II 和 III 组，使用 CMD 解剖了整个角膜厚度的 30%、50%和 70%。对于第 IV、V 和 VI 组，使用 RMD 解剖了整个角膜厚度的 70%、50%和 30%。通过眼前节光学相干断层扫描（ASOCT）检查残余基质厚度，以评估厚度均匀性，并通过扫描电子显微镜（SEM）评估表面质量。