College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China; Wuhan Engineering Research Center of Bee Products on Quality and Safety Control, Wuhan, China.
Hubei Provincial Institute for Food Supervision and Test, Wuhan, China.
Food Res Int. 2022 Apr;154:111014. doi: 10.1016/j.foodres.2022.111014. Epub 2022 Feb 16.
Atherosclerosis (AS) is a serious threat to the health and life of humans worldwide. The mitigating effect of polyphenol compounds from peanut skin extract (PSE) on AS has attracted great research attention. However, the mechanism underlying this mitigating effect remains poorly understood. This study aims to investigate the preventive effect of PSE on high-fat diet-induced AS in mice and explore the underlying mechanisms. PSE treatment significantly reduced atherosclerotic plaques, particularly at high doses. Dietary PSE intervention obviously alleviated the lipid metabolism disorder in ApoE mice by reducing the serum TC and LDL-C contents and increasing the HDL-C content. In addition, PSE intervention significantly decreased the level of pro-inflammatory cytokines TNF-α and IL-6 and increased that of anti-inflammatory IL-10, thus exhibiting a significant anti-inflammatory effect. More interestingly, analysis of the 16S rRNA gene sequence revealed that PSE could significantly alter the community composition of the gut microbiota. Specifically, PSE enhanced the abundance of Roseburia, Rothia, Parabacteroides and Akkermansia, and reduced that of Bilophila and Alistipes. Some of these intestinal bacteria exhibited good anti-inflammatory effects, which are related to the production of short chain fatty acids. Thus, the anti-atherosclerotic effect of PSE may be partly attributed to changes in the composition and function of gut microbiota, which may be closely associated with its anti-inflammatory effect. Moreover, untargeted metabolomics analysis indicated that PSE could regulate the levels of differential metabolites in the liver, serum and fecal samples.
动脉粥样硬化（AS）是全球范围内严重威胁人类健康和生命的疾病。花生衣提取物（PSE）中的多酚化合物对 AS 的缓解作用引起了广泛的研究关注。然而，其缓解作用的机制仍知之甚少。本研究旨在探讨 PSE 对高脂饮食诱导的 AS 小鼠的预防作用及其潜在机制。结果表明，PSE 治疗可显著减少动脉粥样硬化斑块，尤其是高剂量时效果更明显。PSE 干预可通过降低血清 TC 和 LDL-C 含量、增加 HDL-C 含量，明显改善 ApoE 小鼠的脂质代谢紊乱。此外，PSE 干预可显著降低促炎细胞因子 TNF-α和 IL-6 的水平，增加抗炎细胞因子 IL-10 的水平，表现出显著的抗炎作用。更有趣的是，16S rRNA 基因序列分析表明，PSE 可显著改变肠道微生物群落的组成。具体而言，PSE 可增加罗斯伯里氏菌、罗氏菌、副拟杆菌和阿克曼氏菌的丰度，降低双歧杆菌和阿尔斯氏菌的丰度。其中一些肠道细菌具有良好的抗炎作用，这与短链脂肪酸的产生有关。因此，PSE 的抗动脉粥样硬化作用可能部分归因于肠道微生物群落组成和功能的变化，这可能与其抗炎作用密切相关。此外，非靶向代谢组学分析表明，PSE 可调节肝、血清和粪便样本中差异代谢物的水平。
