Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, PR China.
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, PR China.
Bioresour Technol. 2022 May;351:127053. doi: 10.1016/j.biortech.2022.127053. Epub 2022 Mar 23.
A co-fermentation process involving Yarrowia lipolytica and Trichoderma reesei was studied, using distillers grains (DGS) as feedstocks for erythritol production. DGS can be effectively hydrolyzed by cellulase in the single-strain culture of T. reesei. One-pot solid state fermentation for erythritol production was then established by co-cultivating Y. lipolytica M53-S with the 12 h delay inoculated T. reesei Rut C-30, in which efficient saccharification of DGS and improved production of erythritol were simultaneously achieved. The 10:1 inoculation proportion of Y. lipolytica and T. reesei contributed to the maximum erythritol production of 267.1 mg/gds under the optimal conditions including initial moisture of 55%, pH of 5.0, NaCl addition of 0.02 g/gds and DGS mass of 200 g in 144 h co-cultivation. Being compared with the attempts to produce erythritol from other raw materials, the one-pot SSF with DGS is proposed to be a potential strategy for efficient and economical erythritol production.
研究了涉及脂肪假丝酵母和里氏木霉的共发酵过程，以酒糟（DGS）作为生产赤藓糖醇的原料。DGS 可以通过里氏木霉的单菌株培养中的纤维素酶有效水解。然后，通过共培养脂肪假丝酵母 M53-S 与延迟接种 12 小时的里氏木霉 Rut C-30，建立了一锅固态发酵生产赤藓糖醇的方法，其中同时实现了 DGS 的高效糖化和赤藓糖醇的生产提高。在最佳条件下，脂肪假丝酵母和里氏木霉的接种比例为 10:1，初始水分 55%、pH 值 5.0、添加 0.02 g/gds 的 NaCl 和在 144 小时共培养中 200 g DGS 质量，赤藓糖醇的最大产量为 267.1 mg/gds。与从其他原料生产赤藓糖醇的尝试相比，提出了一锅 SSF 用 DGS 是一种高效、经济的生产赤藓糖醇的潜在策略。
