Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, China.
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.
Bioresour Technol. 2017 Nov;244(Pt 1):1089-1095. doi: 10.1016/j.biortech.2017.08.014. Epub 2017 Aug 22.
Okara (soybean residue) is an ideal erythritol feedstock due to its low price and high-nutrient content. Fungal-fermentations were carried out in okara using in-house enzyme pools generated by Mucor flavus or Trichoderma reesei to make okara more accessibility in the subsequent erythritol production using Yarrowia lipolytica. Mucor-fermented okara produced a high erythritol yield because of its special component and micromorphology. Five days is the optimal period for Mucor fermentation. Different fermentation modes were compared in terms of erythritol production and yield. The concentration of Mucor-fermented okara in erythritol fermentation medium was optimal at 30.0g/L. Moreover, 40.0g/L NaCl was added as the osmotic regulator. No extra ingredient was needed during this process. Results of 5-L fermentations showed that an erythritol titer of 14.7g/L, with a yield of 0.49g/g okara was obtained. These findings indicated that Mucor-fermented okara was an economically alternative feedstock for low-cost erythritol production.
豆渣（大豆残渣）是一种理想的赤藓糖醇原料，因为它价格低廉，营养丰富。使用黄曲霉或里氏木霉产生的内部酶制剂池对豆渣进行真菌发酵，以使随后使用解脂耶氏酵母生产赤藓糖醇时，豆渣更易被利用。由于其特殊的成分和微观形态，经过霉菌发酵的豆渣可产生较高的赤藓糖醇产量。霉菌发酵 5 天是最佳时期。从赤藓糖醇生产和产量方面比较了不同的发酵方式。赤藓糖醇发酵培养基中，经过霉菌发酵的豆渣浓度以 30.0g/L 为最佳。此外，添加 40.0g/L 的 NaCl 作为渗透压调节剂。在此过程中不需要添加其他成分。5-L 发酵结果表明，赤藓糖醇的浓度为 14.7g/L，豆渣的得率为 0.49g/g。这些发现表明，经过霉菌发酵的豆渣是一种经济上可行的替代原料，可用于低成本的赤藓糖醇生产。
