Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huaian, China.
Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, China.
Bioresour Technol. 2019 Dec;294:122194. doi: 10.1016/j.biortech.2019.122194. Epub 2019 Sep 25.
Oil crop wastes are attractive feedstocks in microbial processes due to their low cost. However, the product yields can be limited by their undesirable nitrogen surplus. Present study proposed a one-step solid state fermentation (SSF) method for producing erythritol from unrefined oil crop wastes using a modified strain Y. lipolytica M53-S. Enhanced erythritol production (185.4 mg/gds) was obtained from peanut press cake mixed with 40% sesame meal and 10% waste cooking oil. The process was performed at pH 4.0 in 5 L flasks, with initial moisture content, NaCl addition, and inoculum size of 70%, 0.02 g/gds, and 7.5 × 10 cells/gds, respectively. This procedure showed advantages in terms of lower material cost than that of submerged fermentation and shorter culture cycle (96 h) than other SSF processes. In repeated-batch fermentation, erythritol was continuously produced for seven cycles. This study presents a feasible approach in developing an efficient erythritol cultivation from nitrogen-rich wastes.
油料作物废弃物由于成本低，在微生物过程中是很有吸引力的原料。然而，由于其氮素过剩，产物的产量可能会受到限制。本研究提出了一种一步固态发酵（SSF）方法，使用改良的 Y. lipolytica M53-S 菌株从未精炼的油作物废弃物中生产赤藓糖醇。从花生压榨饼与 40%芝麻粉和 10%废食用油混合得到了较高的赤藓糖醇产量（185.4mg/gds）。该过程在 pH 4.0 下，在 5L 摇瓶中进行，初始水分含量、NaCl 添加量和接种量分别为 70%、0.02g/gds 和 7.5×10 个细胞/gds。与深层发酵相比，该方法在材料成本上具有优势，与其他 SSF 工艺相比，培养周期更短（96h）。在重复分批发酵中，赤藓糖醇连续生产了七个周期。本研究提出了一种从富氮废物中高效培养赤藓糖醇的可行方法。
