School of Chemical Engineering & Technology, China University of Mining and Technology, Xuzhou, 221116, Jiangsu, P. R. China.
Analytical Chemistry - Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Universitätsstr. 150, 44780, Bochum, Germany.
Chemistry. 2022 May 25;28(30):e202200868. doi: 10.1002/chem.202200868. Epub 2022 Apr 12.
The use of enzymes as catalysts in chemical synthesis offers advantages in terms of clean and highly selective transformations. Galactose oxidase (GalOx) is a remarkable enzyme with several applications in industrial conversions as it catalyzes the oxidation of primary alcohols. We have investigated the wiring of GalOx with a redox polymer; this enables mediated electron transfer with the electrode surface for its potential application in biotechnological conversions. As a result of electrochemical regeneration of the catalytic center, the formation of harmful H O is minimized during enzymatic catalysis. The introduced bioelectrode was applied to the conversion of bio-renewable platform materials, with glycerol as model substrate. The biocatalytic transformations of glycerol and 5-hydroxymethylfurfural (HMF) were investigated in a circular flow-through setup to assess the possibility of substrate over-oxidation, which is observed for glycerol oxidation but not during HMF conversion.
酶作为化学合成中的催化剂具有清洁和高度选择性转化的优势。半乳糖氧化酶（GalOx）是一种非常有应用前景的酶，它可以催化伯醇的氧化，在工业转化中有多种应用。我们已经研究了 GalOx 与氧化还原聚合物的连接；这使得与电极表面进行中介电子转移成为可能，从而使其在生物技术转化中有潜在的应用。由于催化中心的电化学再生，在酶催化过程中，有害的 H2O2 的形成被最小化。引入的生物电极被应用于生物可再生平台材料的转化，以甘油为模型底物。在循环流动装置中研究了甘油和 5-羟甲基糠醛（HMF）的生物催化转化，以评估底物过度氧化的可能性，这种现象在甘油氧化中观察到，但在 HMF 转化中没有观察到。
