Shi Meihan, Shang Shunlai, Yang Yunzhao, Li Qinggang, Bai Xue-Yuan
School of Medicine, Nankai University, Tianjin 300071, China; Department of Nephrology, The First Medical Center, Chinese PLA General Hospital, Medical School of Chinese PLA, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Department of Nephrology, The First Medical Center, Chinese PLA General Hospital, Medical School of Chinese PLA, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing 100853, China.
Stem Cell Res. 2022 May;61:102760. doi: 10.1016/j.scr.2022.102760. Epub 2022 Mar 22.
Induced pluripotent stem cell (iPSC) lines for studies investigating many diseases can be established from peripheral blood mononuclear cells; here, an iPSC line was established from CD34 cells isolated from the peripheral blood of a healthy woman. The cells were electrotransfected with three different recombinant plasmids to generate a normal-karyotype iPSC line that expresses characteristic surface markers and other pluripotent stem cell genes and can differentiate into all three germ layers in vivo. These newly established iPSC lines, a normal human cell line, can serve as a control line in studies investigating the pathogenesis of various diseases and meet the conditions for organoid studies.
用于研究多种疾病的诱导多能干细胞（iPSC）系可从外周血单个核细胞建立；在此，从一名健康女性外周血中分离出的CD34细胞建立了一个iPSC系。将细胞用三种不同的重组质粒进行电转染，以生成一个具有正常核型的iPSC系，该系表达特征性表面标志物和其他多能干细胞基因，并且能够在体内分化为所有三个胚层。这些新建立的iPSC系作为一种正常人类细胞系，可在研究各种疾病发病机制的研究中用作对照系，并满足类器官研究的条件。
