测量内源性赤霉素和生长素。
Measuring Endogenous GA and IAA.
作者信息
Chen Yunping, Dan Zhiwu, Li Shaoqing
机构信息
State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, China.
出版信息
Bio Protoc. 2022 Feb 20;12(4):e4332. doi: 10.21769/BioProtoc.4332.
Abstract
Plant hormones regulate many physiological processes that largely influence growth, differentiation, and development. Contents of phytohormones were analyzed using a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) system. This protocol describes a detailed procedure to extract and quantify indole-3-acetic acid (IAA) and gibberellin acid (GA) in rice () tissues using high-performance liquid chromatography (HPLC)-based method.
摘要
植物激素调节许多生理过程，这些过程在很大程度上影响生长、分化和发育。使用液相色谱-电喷雾电离-串联质谱（LC-ESI-MS/MS）系统分析植物激素的含量。本方案描述了一种基于高效液相色谱（HPLC）的方法，用于提取和定量水稻（）组织中的吲哚-3-乙酸（IAA）和赤霉素酸（GA）的详细程序。
相似文献
1
Measuring Endogenous GA and IAA.测量内源性赤霉素和生长素。
Bio Protoc. 2022 Feb 20;12(4):e4332. doi: 10.21769/BioProtoc.4332.
2
Quantification of indole-3-acetic acid and amino acid conjugates in rice by liquid chromatography-electrospray ionization-tandem mass spectrometry.利用液相色谱-电喷雾电离-串联质谱法对水稻中吲哚-3-乙酸及其氨基酸共轭物进行定量分析。
Biosci Biotechnol Biochem. 2005 Apr;69(4):778-83. doi: 10.1271/bbb.69.778.
3
Metabolism of indole-3-acetic acid in rice: identification and characterization of N-beta-D-glucopyranosyl indole-3-acetic acid and its conjugates.水稻中吲哚 - 3 - 乙酸的代谢：N-β-D-吡喃葡萄糖基吲哚 - 3 - 乙酸及其共轭物的鉴定与表征
Phytochemistry. 2007 Oct;68(20):2512-22. doi: 10.1016/j.phytochem.2007.05.040. Epub 2007 Jul 12.
4
Profiling of phytohormones in rice under elevated cadmium concentration levels by magnetic solid-phase extraction coupled with liquid chromatography tandem mass spectrometry.利用磁固相萃取结合液相色谱串联质谱法分析镉浓度升高条件下水稻中的植物激素
J Chromatogr A. 2015 Aug 7;1406:78-86. doi: 10.1016/j.chroma.2015.06.046. Epub 2015 Jun 22.
5
Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction.固相萃取后，采用高效液相色谱法和液相色谱-串联质谱法同时分析椰子（Cocos nucifera L.）水中不同种类的植物激素。
Anal Chim Acta. 2008 Mar 10;610(2):274-81. doi: 10.1016/j.aca.2008.01.045. Epub 2008 Jan 25.
6
Simultaneous determination of gibberellic acid, indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry.通过固相萃取和液相色谱-电喷雾串联质谱法同时测定小麦提取物中的赤霉酸、吲哚-3-乙酸和脱落酸。
Talanta. 2008 Aug 15;76(4):798-802. doi: 10.1016/j.talanta.2008.04.041. Epub 2008 Apr 24.
7
Trace analysis of multi-class phytohormones in Oryza sativa using different scan modes in high-resolution Orbitrap mass spectrometry: method validation, concentration levels, and screening in multiple accessions.采用高分辨率轨道阱质谱仪不同扫描模式分析水稻中多类植物激素：方法验证、浓度水平及多份材料的筛选。
Anal Bioanal Chem. 2018 Jul;410(18):4527-4539. doi: 10.1007/s00216-018-1112-9. Epub 2018 May 23.
8
Simultaneous quantification of phytohormones in fermentation extracts of Botryodiplodia theobromae by liquid chromatography-electrospray tandem mass spectrometry.利用液相色谱-电喷雾串联质谱法同时定量测定可可球二孢发酵提取物中的植物激素。
World J Microbiol Biotechnol. 2014 Jul;30(7):1937-46. doi: 10.1007/s11274-014-1612-5. Epub 2014 Feb 9.
9
A liquid chromatography-tandem mass spectrometry-based assay for indole-3-acetic acid-amido synthetase.一种基于液相色谱-串联质谱的吲哚-3-乙酸酰胺合成酶检测方法。
Anal Biochem. 2009 Jul 15;390(2):149-54. doi: 10.1016/j.ab.2009.04.027. Epub 2009 Apr 23.
10
Plant growth promoting potential of butyl isobutyl phthalate and Streptomyces sp. from Rumex dentatus on rice.丁基异丁基邻苯二甲酸酯和弯颈霉属菌对水稻的促生长潜力。
Appl Microbiol Biotechnol. 2022 Apr;106(7):2603-2617. doi: 10.1007/s00253-022-11862-w. Epub 2022 Mar 9.
引用本文的文献
1
A Transformer-LSTM-SVR hybrid model for AI-driven emotional optimization in NEV embedded interior systems.一种用于新能源汽车嵌入式内饰系统中人工智能驱动情感优化的Transformer-LSTM-SVR混合模型。
Sci Rep. 2025 Aug 18;15(1):30236. doi: 10.1038/s41598-025-15808-w.
2
Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids.转录组分析揭示了蝴蝶兰花朵开放过程中花瓣生长的潜在机制。
BMC Plant Biol. 2025 May 24;25(1):696. doi: 10.1186/s12870-025-06713-5.
3
Morphophysiological and transcriptomic analyses during the development of microspores and megaspores in .[物种名称]中小孢子和大孢子发育过程中的形态生理及转录组分析
Front Plant Sci. 2025 Mar 26;16:1540594. doi: 10.3389/fpls.2025.1540594. eCollection 2025.
4
Auxin regulates bulbil initiation by mediating sucrose metabolism in .生长素通过介导[具体部位]中的蔗糖代谢来调节珠芽起始。
Hortic Res. 2024 Feb 23;11(4):uhae054. doi: 10.1093/hr/uhae054. eCollection 2024 Apr.
本文引用的文献
1
Rice controls tiller number by regulating strigolactone synthesis.水稻通过调控独脚金内酯的合成来控制分蘖数。
Plant Signal Behav. 2020 Nov 1;15(11):1804685. doi: 10.1080/15592324.2020.1804685. Epub 2020 Aug 11.
2
Modulates Plant Architecture through Regulating GA and Indole-3-Acetic Acid Metabolism.通过调节赤霉素和吲哚-3-乙酸代谢来调节植物的结构。
Plant Physiol. 2020 Sep;184(1):393-406. doi: 10.1104/pp.20.00302. Epub 2020 Jun 24.
3
Phytohormones signaling and crosstalk regulating leaf angle in rice.植物激素信号传导及相互作用对水稻叶夹角的调控
Plant Cell Rep. 2016 Dec;35(12):2423-2433. doi: 10.1007/s00299-016-2052-5. Epub 2016 Sep 13.
4
Determination of abscisic acid and its glucosyl ester in embryogenic callus cultures of Vitis vinifera in relation to the maturation of somatic embryos using a new liquid chromatography-ELISA analysis method.采用一种新的液相色谱-酶联免疫吸附分析方法，测定葡萄胚性愈伤组织培养物中脱落酸及其葡萄糖基酯与体细胞胚成熟的关系。
J Plant Physiol. 2014 Jun 15;171(10):852-9. doi: 10.1016/j.jplph.2014.01.014. Epub 2014 Feb 23.
5
Measurement of plant hormones by liquid chromatography-mass spectrometry.采用液相色谱-质谱联用技术测定植物激素。
Methods Mol Biol. 2009;495:39-51. doi: 10.1007/978-1-59745-477-3_4.
Zotero 插件