以嗜肺军团菌全细胞为抗原的酶联免疫吸附测定。用于人类军团菌病血清诊断的单价抗原与多价抗原的比较。

ELISA using whole Legionella pneumophila cell as antigen. Comparison between monovalent and polyvalent antigens for the serodiagnosis of human legionellosis.

作者信息

Barka N, Tomasi J P, Stadtsbaeder S

出版信息

J Immunol Methods. 1986 Oct 23;93(1):77-81. doi: 10.1016/0022-1759(86)90435-7.

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) using the six serogroups of whole L. pneumophila bound to microtitre plate wells is described for the serodiagnosis of legionellosis. Comparative studies using monovalent antigen indicated a high correlation between ELISA and indirect immunofluorescence antibody (IFA) tests (r = 0.90, P less than 0.001). Testing 196 human sera by ELISA using both monovalent and polyvalent antigens have established the efficiency of the polyvalent antigen for screening purposes. The ELISA test system exhibited rapidity, sensitivity and reproducibility and should be considered as an alternative to the IFA test for routine serodiagnosis of legionellosis.

摘要

本文描述了一种间接酶联免疫吸附测定（ELISA）方法，该方法使用与微量滴定板孔结合的嗜肺军团菌六个血清群进行军团病的血清学诊断。使用单价抗原的比较研究表明，ELISA与间接免疫荧光抗体（IFA）试验之间具有高度相关性（r = 0.90，P < 0.001）。使用单价和多价抗原通过ELISA检测196份人血清，确定了多价抗原用于筛查的有效性。ELISA检测系统具有快速、灵敏和可重复性，应被视为军团病常规血清学诊断中IFA检测的替代方法。