通过微量酶联免疫吸附测定和免疫荧光测定法检测抗嗜肺军团菌血清群1至6以及莱顿-1菌株的抗体。
Detection of antibodies against Legionella pneumophila serogroups 1 to 6 and the Leiden-1 strain by micro ELISA and immunofluorescence assay.
作者信息
Herbrink P, Meenhorst P L, Groothuis D G, Munckhof H V, Bax R, Meijer C J, Lindeman J
出版信息
J Clin Pathol. 1983 Nov;36(11):1246-52. doi: 10.1136/jcp.36.11.1246.
One hundred and seventy-five human sera were tested for the presence of type-specific antibodies against L pneumophila serogroups 1 to 6 and the Leiden-1 strain by means of an enzyme linked immunosorbent assay (ELISA) and compared with the results obtained by the indirect immunofluorescence assay (IFA). A high correlation (correlation coefficient 0.92) between both methods was found. No consistent pattern of IgG, IgA and IgM classes of antibody titres against L pneumophila were found. In the sera of 15 of 17 patients with a proven L pneumophila pneumonia, IgM class antibodies against L pneumophila could be detected. A "polyvalent" ELISA was developed which permits rapid routine screening of human sera for antibodies against L pneumophila serogroups 1 to 6 and the Leiden-1 strain.
采用酶联免疫吸附测定（ELISA）检测了175份人血清中针对嗜肺军团菌血清型1至6以及莱顿-1菌株的型特异性抗体，并与间接免疫荧光测定（IFA）的结果进行了比较。发现两种方法之间具有高度相关性（相关系数为0.92）。未发现针对嗜肺军团菌的IgG、IgA和IgM类抗体滴度的一致模式。在17例确诊为嗜肺军团菌肺炎的患者中，有15例患者的血清中可检测到针对嗜肺军团菌的IgM类抗体。开发了一种“多价”ELISA，可用于快速常规筛查人血清中针对嗜肺军团菌血清型1至6以及莱顿-1菌株的抗体。
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