类器官共培养方法捕获癌细胞-自然杀伤细胞相互作用。
Organoid Co-culture Methods to Capture Cancer Cell-Natural Killer Cell Interactions.
机构信息
Division of Hematology and Oncology, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.
出版信息
Methods Mol Biol. 2022;2463:235-250. doi: 10.1007/978-1-0716-2160-8_17.
Metastasis is a complex process that has been historically difficult to model in culture. Host immune responses play critical roles in restraining and promoting metastatic tumor cells. Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor organoids to capture interactions between the two cellular populations. These assays can be used to model key aspects of metastatic biology and to screen for the effectiveness of agents that stimulate natural killer cell cytotoxicity.
转移是一个复杂的过程，在培养皿中进行建模一直具有挑战性。宿主的免疫反应在抑制和促进转移性肿瘤细胞方面起着关键作用。在这里，我们描述了一种自然杀伤细胞和肿瘤类器官的 3D 器官型共培养方法，以捕获这两种细胞群之间的相互作用。这些测定可用于模拟转移生物学的关键方面，并筛选刺激自然杀伤细胞细胞毒性的药物的效果。
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