甜菜根提取物通过靶向肿瘤微环境中的免疫反应克服新辅助蒽环类环磷酰胺方案的化疗耐药性的作用：乳腺腺癌大鼠的临床前研究。

The Role of Beetroot Extract in Overcoming Chemoresistance of Neoadjuvant Adriamycin Cyclophosphamide Regimen by Targeting Immune Response in Tumor Microenvironment: A Preclinical Study in Mammary Adenocarcinoma Rats.

机构信息

Doctoral Program of Medical and Health Sciences, Faculty of Medicine, Diponegoro University, Semarang, Indonesia.

Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Wahid Hasyim University, Semarang, Indonesia.

出版信息

Asian Pac J Cancer Prev. 2022 Mar 1;23(3):1061-1068. doi: 10.31557/APJCP.2022.23.3.1061.

OBJECTIVE

This study aims to determine the role of beetroot extract in overcoming the chemoresistance of Neoadjuvant Adriamycin Cyclophosphamide (NAC) regimens with a target immune response in the tumour microenvironment at the pre-clinical stage.

METHODS

This study was conducted on rats with 7,12-Dimethyl Benz (α) Anthracene (DMBA) induced mammary adenocarcinoma. Adriamycin Cyclophosphamide was given in 4 cycles, whereas beetroot extract was administered three times each cycle. Observations of CD8 T cells and Myeloid Derivative Suppressive Cells (MDSC) expression levels and pathological responses were carried out on tumour tissue taken at the end of the observation.

RESULTS

Supplementation of beetroot extract to NAC could significantly increase CD8 T cells and decrease MDSC in the tumour microenvironment. The addition of beetroot extract gave a better pathological response.

CONCLUSION

Beetroot extract enhances the immune response in the tumor microenvironment so that it has the potential to overcome chemoresistance in NAC.
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目的

本研究旨在确定甜菜根提取物在临床前阶段通过靶向肿瘤微环境中的免疫反应来克服新辅助多柔比星环磷酰胺（NAC）方案的化疗耐药性中的作用。
方法：本研究在 7,12-二甲基苯（α）蒽（DMBA）诱导的乳腺腺癌大鼠中进行。给予多柔比星环磷酰胺 4 个周期，而甜菜根提取物每个周期给予 3 次。在观察结束时取肿瘤组织进行 CD8 T 细胞和髓系衍生抑制细胞（MDSC）表达水平和病理反应的观察。
结果：NAC 中甜菜根提取物的补充可以显著增加肿瘤微环境中的 CD8 T 细胞并减少 MDSC。添加甜菜根提取物可获得更好的病理反应。
结论：甜菜根提取物增强了肿瘤微环境中的免疫反应，因此有可能克服 NAC 的化疗耐药性。