熊去氧胆酸偶联壳聚糖用于 HuCC-T1 人胆管癌细胞的光动力治疗。
Ursodeoxycholic acid-conjugated chitosan for photodynamic treatment of HuCC-T1 human cholangiocarcinoma cells.
机构信息
National Research & Development Center for Hepatobiliary Cancer, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Gyeongnam 626-770, Republic of Korea.
出版信息
Int J Pharm. 2013 Sep 15;454(1):74-81. doi: 10.1016/j.ijpharm.2013.06.035. Epub 2013 Jul 6.
Chitosan was hydrophobically modified with ursodeoxycholic acid (UDCA) to fabricate nano-photosensitizer for photodynamic therapy (PDT) of HuCC-T1 cholangiocarcinoma cells. Synthesis of UDCA-conjugated chitosan (ChitoUDCA) was confirmed using (1)H NMR spectra. Chlorin E6 (Ce6) was used as a photosensitizer and incorporated into ChitoUDCA nanoparticles through formation of ion complexes. Morphology of Ce6-incorporated ChitoUDCA nanoparticles was observed using TEM and their shapes were spherical with sizes around 200-400 nm. The PDT potential of Ce6-incorporated ChitoUDCA nanoparticles were studied with HuCC-T1 human cholangiocarcinoma cells. The results showed that ChitoUDCA nanoparticles enhances of Ce6 uptake into tumor cells, phototoxicity, and ROS generation compared to Ce6 itself. Furthermore, Ce6-incorporated ChitoUDCA nanoparticles showed quenching in aqueous solution and sensing at tumor cells. We suggest that Ce6-incorporated ChitoUDCA nanoparticles are promising candidates for PDT of cholangiocarcinoma cells.
壳聚糖被熊去氧胆酸（UDCA）疏水改性，以制备用于胆管癌细胞光动力治疗（PDT）的纳米光敏剂。使用（1）H NMR 谱证实了熊去氧胆酸接枝壳聚糖（ChitoUDCA）的合成。氯乙酮（Ce6）被用作光敏剂，并通过形成离子复合物掺入 ChitoUDCA 纳米颗粒中。使用 TEM 观察了掺入 Ce6 的 ChitoUDCA 纳米颗粒的形态，其形状为球形，尺寸约为 200-400nm。用 HuCC-T1 人胆管癌细胞研究了掺入 Ce6 的 ChitoUDCA 纳米颗粒的 PDT 潜力。结果表明，与 Ce6 本身相比，ChitoUDCA 纳米颗粒增强了 Ce6 进入肿瘤细胞的摄取、光毒性和 ROS 生成。此外，掺入 Ce6 的 ChitoUDCA 纳米颗粒在水溶液中显示出猝灭，并在肿瘤细胞中进行传感。我们认为，掺入 Ce6 的 ChitoUDCA 纳米颗粒是胆管癌细胞 PDT 的有前途的候选物。
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