Department of Physiology, Faculty of Veterinary Medicine, Mustafa Kemal University, Antakya, Türkiye.
Department of Surgery, Faculty of Veterinary Medicine, Mustafa Kemal University, Antakya, Türkiye.
J Food Biochem. 2022 Aug;46(8):e14161. doi: 10.1111/jfbc.14161. Epub 2022 Mar 29.
The protective effects of the ethanol extract of Smilax excelsa L. (SE) leaves were investigated on testicular tissue of rats with a torsion model in this study. The chemical composition of the extract was detected by means of liquid chromatography with tandem mass spectrometry (LC-MS/MS). SE extract was given for 21 days before torsion was created in the treatment group. The sperm parameters of the torsion group were impaired, and there was an increase in MDA level as well as a decrease in GSH level and GPx activity compared to the control group. TNF-α and NF-κB levels in the torsion group increased as compared to those in the control group. The expression levels of Nrf-2 and HO-1 were lower in the torsion group than those in the control group. The SE pretreatment group has improved sperm, oxidative stress, and inflammatory markers when compared to the torsion group, and the Nrf-2/HO-1 pathway was activated. PRACTICAL APPLICATIONS: Smilax excelsa L. is a plant with economic value used in traditional medicine in the treatment of stomachache, bloating, and breast cancer in Northwest Anatolia. It has an antioxidant effect due to the flavonoids and anthocyanins it contains. The protective effect against ischemia-reperfusion-induced tissue and reproductive damage in testicular tissue were demonstrated with the study. When the histological examinations of the tissues were evaluated, it was found that morphological structure of the tissues was retained in the treatment group. The findings indicate that SE prevents tissue damage in the torsion model by antioxidant and anti-inflammatory effects and activating Nrf-2/HO-1 pathway.
本研究旨在探讨菝葜叶乙醇提取物（SE）对扭转模型大鼠睾丸组织的保护作用。采用液相色谱-串联质谱（LC-MS/MS）检测提取物的化学成分。在造模前 21 天，治疗组给予 SE 提取物。与对照组相比，扭转组的精子参数受损，MDA 水平升高，GSH 水平和 GPx 活性降低，TNF-α和 NF-κB 水平升高。与对照组相比，扭转组的 Nrf-2 和 HO-1 表达水平较低。与扭转组相比，SE 预处理组的精子、氧化应激和炎症标志物得到改善，Nrf-2/HO-1 通路被激活。
菝葜 L. 是一种具有经济价值的植物，在安纳托利亚西北部的传统医学中用于治疗胃痛、腹胀和乳腺癌。由于其含有的类黄酮和花青素，它具有抗氧化作用。该研究证明了它对缺血再灌注引起的睾丸组织损伤和生殖损伤的保护作用。当评估组织的组织学检查时，发现治疗组保留了组织的形态结构。研究结果表明，SE 通过抗氧化和抗炎作用以及激活 Nrf-2/HO-1 通路来防止扭转模型中的组织损伤。
