Zhang Yuanxin, Shi Huiyu, Yun Yanhong, Feng Haibo, Wang Xuemei
College of Animal Science and Technology, Hainan University, Haikou 570228, China.
Animals (Basel). 2022 Nov 28;12(23):3320. doi: 10.3390/ani12233320.
(1) Background: The mammary glands of the perinatal goats are susceptible to reactive oxygen species (ROS) leading to oxidative injury. Although L. is rich in anthocyanins with high safety and excellent free-radical-scavenging ability, the effect and mechanism of L. anthocyanins (DAC) on the antioxidant capacity of the black Hainan goat has been the subject of few studies to date; (2) Methods: For this reason, feeding experiments were performed by feeding experimental diets, and the pre-protective capacity of DAC on goat mammary epithelial cells was explored on the basis of the established model of HO injury; (3) Results: As well as altering rumen fermentation parameters in perinatal female goats, dietary challenge also improves antioxidant capacity in their blood and milk. thereby enhancing children's antioxidant capacity and increasing their resistance to oxidative stress. However, we also found that DAC pretreatment was capable of activating both Nrf2 and MAPK/JNK pathways, which results in enhanced antioxidase activity and elimination of ROS; (4) Conclusions: Together, these findings suggest that DAC may have a pre-protective role on perinatal Hainan black goats through the regulation of Nrf2 and MAPK/JNK pathways in GMEC.
(1) 背景：围产期山羊的乳腺易受活性氧（ROS）影响，导致氧化损伤。尽管[具体名称未给出]富含花青素，具有高安全性和出色的自由基清除能力，但迄今为止，[具体名称未给出]花青素（DAC）对海南黑山羊抗氧化能力的影响及机制鲜有研究；(2) 方法：因此，通过投喂实验日粮进行饲养实验，并在已建立的HO损伤模型基础上，探究DAC对山羊乳腺上皮细胞的预保护能力；(3) 结果：日粮处理不仅改变了围产期母羊的瘤胃发酵参数，还提高了其血液和乳汁中的抗氧化能力，从而增强了子代的抗氧化能力，提高了它们对氧化应激的抵抗力。然而，我们还发现DAC预处理能够激活Nrf2和MAPK/JNK途径，从而增强抗氧化酶活性并清除ROS；(4) 结论：综上所述，这些发现表明DAC可能通过调节GMEC中的Nrf2和MAPK/JNK途径，对围产期海南黑山羊起到预保护作用。
