Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 181 Ipsingil, Jeongeup, Jeonbuk 56212, Republic of Korea.
University of Science and Technology (UST), 217 Gajeong-ro, Yuseong, Daejeon 34113, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005296.
A Gram-stain-positive, non-spore-forming, yellow-pigmented, non-motile, non-flagellated, facultative anaerobic and rod-shaped bacterial strain, designated BI34, was isolated from the gut of the larva of . Strain BI34 grew at 15-40 °C (optimum, 37 °C), at pH 6.5-9.0 (optimum, pH 7.5) and in the presence of 0-7 % (w/v) NaCl (optimum, 2 %). Based on the results of 16S rRNA gene sequence analysis, strain BI34 belonged to the phylum and was closely related to NBRC 104118 (99.3 %), NBRC 15516 (99.1 %), BIT-GX5 (99.0 %), SE3 (99.0 %), NBRC 110994 (98.4 %) and DS-61 (97.0 %). The genome to genome relatedness of the average nucleotide identity (ANI) and the digital DNA-DNA hybridization (dDDH) values calculated by the Genome-to-Genome Distance Calculator between strain BI34 and its related species mentioned above were lower than the threshold of 95 and 70 % for speciation, respectively. The predominant menaquinone of strain BI34 contained MK-9(H), and the major fatty acids were anteiso-C, C and anteiso-C. Strain BI34 had diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. The whole-cell sugars were galactose, glucose and ribose, and the cell-wall peptidoglycan contained lysine, alanine, aspartic acid and glutamic acid. The DNA G+C content of strain BI34 was 73.8 mol%. The difference in physiological and biochemical characteristics and the below-threshold values of genome-to-genome relatedness indicate that strain BI34 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is BI34 (=KCTC 49302=NBRC 114073).
一株革兰氏阳性、非孢子形成、黄色色素、非运动、无鞭毛、兼性厌氧、棒状的细菌菌株，被命名为 BI34，从幼虫的肠道中分离出来。BI34 菌株在 15-40°C（最佳温度为 37°C）、pH 值 6.5-9.0（最佳 pH 值为 7.5）和 0-7%（w/v）NaCl（最佳为 2%）的条件下生长。基于 16S rRNA 基因序列分析的结果，BI34 菌株属于 ，与 NBRC 104118（99.3%）、NBRC 15516（99.1%）、BIT-GX5（99.0%）、SE3（99.0%）、NBRC 110994（98.4%）和 DS-61（97.0%）最为密切相关。通过基因组到基因组距离计算器计算的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值的基因组到基因组相关性，BI34 菌株与其上述相关种之间的数值均低于种形成的阈值 95%和 70%。BI34 菌株的主要menaquinone 含有 MK-9(H)，主要脂肪酸为 anteiso-C、C 和 anteiso-C。BI34 菌株的主要极性脂为双磷脂酰甘油和磷脂酰甘油。全细胞糖为半乳糖、葡萄糖和核糖，细胞壁肽聚糖含有赖氨酸、丙氨酸、天冬氨酸和谷氨酸。BI34 菌株的 DNA G+C 含量为 73.8mol%。生理生化特性的差异和基因组到基因组相关性的阈值以下表明，BI34 菌株代表了属中的一个新种，因此建议将其命名为 sp. nov.。其模式菌株为 BI34（=KCTC 49302=NBRC 114073）。
