Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Department of Biology, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004905.
A Gram-stain-positive, yellow-pigmented, non-motile actinobacterial strain, designated as BIT-GX5, was isolated from a sesame husks compost collected in Beijing, PR China. This bacterium was found to be able to grow in the temperature range from 16 to 50 °C and had an optimal growth temperature at 45 °C. Its taxonomic position was analysed using a polyphasic approach. The 16S rRNA gene sequence (1482 bp) of strain BIT-GX5 was most similar to ATCC BAA-886 (99.45%), LMG 16121 (99.17%) and RS-7-4 (98.75%). The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of five housekeeping genes (, , , and ) and genome sequences, placed strain BIT-GX5 in a separate lineage among the genus within the family . The major polar lipids of strain BIT-GX5 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid and aminolipid. The major isoprenoid quinone was MK-9(H), while the cell-wall sugars were galactose, rhamnose, glucose and mannose. The peptidoglycan type was A4α l-Lys-d-Ser-d-Asp. The major fatty acids were anteiso-C and iso-C, which were similar to other members in the genus Results of DNA-DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests exhibited the genotypic and phenotypic differentiation of strain BIT-GX5 from the other members of the genus . Therefore, strain BIT-GX5 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BIT-GX5 (= CGMCC 1.17687 = KCTC 49391).
一株革兰氏阳性、黄色、非运动放线菌菌株，被命名为 BIT-GX5，从中国北京采集的芝麻壳堆肥中分离得到。该细菌被发现能够在 16 到 50°C 的温度范围内生长，最适生长温度为 45°C。采用多相分析方法对其分类地位进行了分析。菌株 BIT-GX5 的 16S rRNA 基因序列（1482 bp）与 ATCC BAA-886（99.45%）、LMG 16121（99.17%）和 RS-7-4（98.75%）最为相似。基于 16S rRNA 基因、五个看家基因（、、、和 ）的串联序列和基因组序列的系统发育分析结果，将菌株 BIT-GX5 置于 属内的一个单独分支中。菌株 BIT-GX5 的主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、氨基磷脂和氨基脂。主要的异戊烯醌为 MK-9(H)，而细胞壁糖为半乳糖、鼠李糖、葡萄糖和甘露糖。肽聚糖类型为 A4α l-Lys-d-Ser-d-Asp。主要脂肪酸为 anteiso-C 和 iso-C，与属内其他成员相似。DNA-DNA 杂交和平均核苷酸同一性计算以及生理生化试验结果表明，菌株 BIT-GX5 与属内其他成员在基因型和表型上存在分化。因此，菌株 BIT-GX5 被认为代表了属内的一个新种，建议命名为 sp. nov.。其模式菌株为 BIT-GX5（=CGMCC 1.17687=KCTC 49391）。
