Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2300 RA Leiden, Netherlands.
Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstraße 1, 39106 Magdeburg, Germany.
Glycobiology. 2022 Jun 13;32(7):580-587. doi: 10.1093/glycob/cwac021.
The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative to standardize the reporting of glycoanalytical methods and to assess their reproducibility. To date, the MIRAGE Commission has published several reporting guidelines that describe what information should be provided for sample preparation methods, mass spectrometry methods, liquid chromatography analysis, exoglycosidase digestions, glycan microarray methods, and nuclear magnetic resonance methods. Here, we present the first version of reporting guidelines for glyco(proteo)mics analysis by capillary electrophoresis (CE) for standardized and high-quality reporting of experimental conditions in the scientific literature. The guidelines cover all aspects of a glyco(proteo)mics CE experiment including sample preparation, CE operation mode (CZE, CGE, CEC, MEKC, cIEF, cITP), instrument configuration, capillary separation conditions, detection, data analysis, and experimental descriptors. These guidelines are linked to other MIRAGE guidelines and are freely available through the project website https://www.beilstein-institut.de/en/projects/mirage/guidelines/#ce_analysis (doi:10.3762/mirage.7).
糖组学实验最低信息要求 (MIRAGE) 是一项旨在规范糖分析方法报告并评估其重现性的计划。迄今为止，MIRAGE 委员会已经发布了几项报告指南，描述了应提供哪些信息用于样品制备方法、质谱方法、液相色谱分析、外切糖苷酶消化、糖基化微阵列方法和核磁共振方法。在这里，我们提出了毛细管电泳 (CE) 糖组学 (糖蛋白组学) 分析的第一个报告指南版本，用于在科学文献中标准化和高质量地报告实验条件。这些指南涵盖了糖组学 CE 实验的所有方面，包括样品制备、CE 操作模式 (CZE、CGE、CEC、MEKC、cIEF、cITP)、仪器配置、毛细管分离条件、检测、数据分析和实验描述符。这些指南与其他 MIRAGE 指南相关联，并可通过项目网站 https://www.beilstein-institut.de/en/projects/mirage/guidelines/#ce_analysis (doi:10.3762/mirage.7) 免费获取。
