Simpson J V, Wiatr C L
National Corn-to-Ethanol Research Center (NCERC), Southern Illinois University-Edwardsville, Edwardsville, IL, United States.
Chemir Analytical Lab, Pfizer, St. Louis, MO, United States.
World J Microbiol Biotechnol. 2022 Mar 29;38(5):82. doi: 10.1007/s11274-022-03253-0.
2,2-Dibromo-3-nitrilopropionamide (DBNPA) has been used as a biocide in industrial water applications due to its instantaneous antimicrobial activity and rapid chemical breakdown. In this study, DBNPA is considered a potential alternative for antibiotics used for bacterial control during corn-to-ethanol fermentation. A method using LC/MS/MS was developed to accurately quantify DBNPA in water. When this method was applied to quantify DBNPA concentration in a fermentation matrix, DBNPA was found to be unstable and to decay rapidly, preventing validation of the method or quantitation. This method was then used to evaluate the degradation rate of DBNPA in whole stillage, which is the nonvolatile residue produced by removal of ethanol from corn-based fermentation beer by distillation through the relative decrease in measured signal. In addition, a method was developed and validated to quantify bromide, one of the degradation products of DBNPA, in whole stillage using LC/MS/MS. The degradation rate of DBNPA in whole stillage was found to display first-order kinetics with a calculated half-life of 85 min. Laboratory analytical chemistry results on DBNPA degradation were confirmed based on a bacterial viability assay in field trials.
2,2 - 二溴 - 3 - 腈基丙酰胺（DBNPA）因其具有即时抗菌活性和快速化学分解的特性，已被用作工业用水中的杀生剂。在本研究中，DBNPA被视为玉米制乙醇发酵过程中用于控制细菌的抗生素的潜在替代品。开发了一种使用液相色谱/串联质谱（LC/MS/MS）的方法来准确测定水中的DBNPA。当将该方法应用于定量发酵基质中的DBNPA浓度时，发现DBNPA不稳定且迅速降解，从而无法对该方法进行验证或定量。然后使用该方法通过测量信号的相对降低来评估DBNPA在全酒糟中的降解速率，全酒糟是通过蒸馏从玉米基发酵啤酒中去除乙醇后产生的不挥发残余物。此外，还开发并验证了一种使用LC/MS/MS定量全酒糟中DBNPA降解产物之一溴化物的方法。发现DBNPA在全酒糟中的降解速率呈现一级动力学，计算得出的半衰期为85分钟。基于田间试验中的细菌活力测定，证实了实验室分析化学关于DBNPA降解的结果。
