State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, 200032, China.
Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, 230032, China.
Neurosci Bull. 2022 Sep;38(9):992-1006. doi: 10.1007/s12264-022-00842-9. Epub 2022 Mar 27.
Reduced levels of retinal dopamine, a key regulator of eye development, are associated with experimental myopia in various species, but are not seen in the myopic eyes of C57BL/6 mice, which are deficient in melatonin, a neurohormone having extensive interactions with dopamine. Here, we examined the relationship between form-deprivation myopia (FDM) and retinal dopamine levels in melatonin-proficient CBA/CaJ mice. We found that these mice exhibited a myopic refractive shift in form-deprived eyes, which was accompanied by altered retinal dopamine levels. When melatonin receptors were pharmacologically blocked, FDM could still be induced, but its magnitude was reduced, and retinal dopamine levels were no longer altered in FDM animals, indicating that melatonin-related changes in retinal dopamine levels contribute to FDM. Thus, FDM is mediated by both dopamine level-independent and melatonin-related dopamine level-dependent mechanisms in CBA/CaJ mice. The previously reported unaltered retinal dopamine levels in myopic C57BL/6 mice may be attributed to melatonin deficiency.
视网膜多巴胺水平降低，这是眼睛发育的关键调节剂，与各种物种的实验性近视有关，但在缺乏褪黑素的 C57BL/6 小鼠的近视眼中并未出现，褪黑素是一种与多巴胺广泛相互作用的神经激素。在这里，我们研究了形态剥夺性近视（FDM）与视网膜多巴胺水平之间的关系在褪黑素功能正常的 CBA/CaJ 小鼠中。我们发现，这些小鼠在形态剥夺眼中表现出近视性屈光变化，同时伴有视网膜多巴胺水平的改变。当用药物阻断褪黑素受体时，仍能诱导 FDM，但幅度减小，并且 FDM 动物的视网膜多巴胺水平不再改变，表明与褪黑素相关的视网膜多巴胺水平变化有助于 FDM。因此，在 CBA/CaJ 小鼠中，FDM 是由多巴胺水平非依赖性和褪黑素相关的多巴胺水平依赖性机制介导的。以前报道的 C57BL/6 小鼠近视时视网膜多巴胺水平不变可能归因于褪黑素缺乏。
