Riddet Institute, Massey University, Palmerston North, New Zealand.
Division of Human Nutrition & Health, Wageningen University & Research, Wageningen, The Netherlands.
J Nutr. 2022 Jul 6;152(7):1635-1646. doi: 10.1093/jn/nxac077.
It is not feasible to determine the true ileal amino acid (AA) digestibility of protein sources in humans on a routine basis, and the growing pig has been recommended as an animal model for this purpose but requires further validation.
To determine and compare true ileal AA digestibility between adult human ileostomates and growing cannulated pigs for a range of food proteins.
Seven protein sources (black beans, bread, collagen, pigeon peas, wheat bran, whey protein isolate, and zein) that spanned the range of digestibilities typically seen in foods were evaluated. Six female growing pigs received each of the protein sources, as well as a protein-free diet, and digesta were collected via ileal T-cannula. Adult human ileostomates consumed the same protein sources (5-8 ileostomates, depending on the protein source), as well as a protein-free diet, and digesta were collected. Titanium dioxide and celite were included in the diets as indigestible markers. True ileal AA digestibility coefficients were determined.
There was a significant effect of protein source (P ≤ 0.001) for all AAs. The effect of species was not significant (P > 0.05) except for total lysine (but not for available lysine). When analyzed within diets, the statistically significant species effect for true lysine digestibility was found for black beans only. Pig and human digestibility values were generally highly and significantly (P ≤ 0.05) correlated. A linear regression equation derived for true ileal AA digestibility (given as coefficients) determined in the human and pig for the overall mean of all AAs was (y = human, x = pig) y = 1.00x - 0.010, with the slope not statistically significant (P > 0.05) from unity and the intercept not different (P > 0.05) from zero.
True ileal AA digestibility values determined in the growing pig can be directly used for predicting digestibility in adult humans.
在常规基础上确定蛋白质源在人体中的真正回肠氨基酸（AA）消化率是不可行的，并且已经推荐生长猪作为该目的的动物模型，但需要进一步验证。
确定并比较成人回肠造口术患者和生长的套管猪对一系列食物蛋白质的真正回肠 AA 消化率。
评估了七种蛋白质源（黑豆、面包、胶原蛋白、兵豆、麦麸、乳清蛋白分离物和玉米蛋白），这些蛋白质源涵盖了食物中常见的消化率范围。六头雌性生长猪接受了每种蛋白质源以及无蛋白质饮食，通过回肠 T 套管收集消化物。成人回肠造口术患者食用了相同的蛋白质源（取决于蛋白质源，5-8 名回肠造口术患者）以及无蛋白质饮食，并收集消化物。二氧化钛和硅藻土作为不可消化的标记物包含在饮食中。确定真正的回肠 AA 消化率系数。
所有 AA 的蛋白质源（P≤0.001）均有显著影响。种属的影响不显著（P>0.05），除了总赖氨酸（但不是有效赖氨酸）。在饮食内分析时，仅在黑豆中发现真正赖氨酸消化率的统计学上显著种属效应。猪和人类的消化率值通常高度且显著（P≤0.05）相关。在人类和猪中确定的所有 AA 的总体平均值的真正回肠 AA 消化率（表示为系数）的线性回归方程为（y=人类，x=猪）y=1.00x-0.010，斜率与 1 无统计学差异（P>0.05），截距与 0 无统计学差异（P>0.05）。
在生长猪中确定的真正回肠 AA 消化率值可直接用于预测成人的消化率。
