Fanelli Natalia S, Martins Juliana C F R, Stein Hans H
Division of Nutritional Sciences, University of Illinois, Urbana, IL, USA.
Department of Animal Sciences, University of Illinois, Urbana, IL, USA.
J Nutr Sci. 2024 Nov 12;13:e68. doi: 10.1017/jns.2024.71. eCollection 2024.
The objectives of this experiment were to determine the digestible indispensable amino acid score (DIAAS) for eggs cooked in different forms and in traditional egg-bread or egg-hash brown combinations, and to test the hypothesis that DIAAS in eggs is greater than in breads or potatoes. Nine ileal cannulated gilts (average initial body weight: 51.1 ± 6.0 kg) were allotted to a 9 × 6 Youden square design with nine diets and six 7-day periods. Fried egg, boiled egg, scrambled egg, English muffin, Texas toast, and hash brown were included in the experiment. Six diets each contained one source of protein and three diets were combinations of fried eggs and English muffin, boiled eggs and Texas toast, or scrambled egg and hash brown. A nitrogen-free diet was also used and fed to all pigs in one period. The standardised ileal digestibility (SID) of crude protein (CP) and amino acids (AA) was calculated, and DIAAS was calculated for the individual ingredient and combined meals for children between 6 and 36 months and individuals older than 3 years. For both age groups, all cooked eggs had greater (P < 0.001) DIAAS compared with the other foods, and hash brown had greater (P < 0.001) DIAAS than both breads. All combined meals had DIAAS greater than 75 and there were no differences between measured and predicted DIAAS for the combined meals. In conclusion, eggs have 'excellent' protein quality for individuals older than 6 months and can compensate for the lower protein quality in plant-based foods, and DIAAS obtained from individual ingredients are additive in mixed meals.
本实验的目的是确定不同烹饪方式的鸡蛋以及传统鸡蛋面包或鸡蛋土豆煎饼组合的可消化必需氨基酸评分(DIAAS),并验证鸡蛋的DIAAS高于面包或土豆的假设。将9头回肠造瘘小母猪(平均初始体重:51.1±6.0千克)分配到一个9×6的尤登方设计中,采用9种日粮和6个7天周期。实验中包括煎蛋、煮蛋、炒蛋、英式松饼、德州吐司和土豆煎饼。六种日粮每种包含一种蛋白质来源,三种日粮是煎蛋和英式松饼、煮蛋和德州吐司或炒蛋和土豆煎饼的组合。还使用了一种无氮日粮,并在一个周期内喂给所有猪。计算了粗蛋白(CP)和氨基酸(AA)的标准化回肠消化率(SID),并计算了6至36个月儿童和3岁以上个体的单一成分和混合餐的DIAAS。对于两个年龄组,与其他食物相比,所有烹饪方式的鸡蛋都具有更高(P<0.001)的DIAAS,并且土豆煎饼的DIAAS高于两种面包(P<0.001)。所有混合餐的DIAAS均大于75,并且混合餐的实测DIAAS与预测DIAAS之间没有差异。总之,对于6个月以上的个体,鸡蛋具有“优质”的蛋白质质量,可以弥补植物性食物中较低的蛋白质质量,并且从单一成分获得的DIAAS在混合餐中具有可加性。