Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran 14115-336, Iran.
Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran 14115-336, Iran.
Poult Sci. 2022 May;101(5):101795. doi: 10.1016/j.psj.2022.101795. Epub 2022 Feb 23.
Fertility potential in roosters is a crucial topic in broiler breeder reproduction which is thought to be associated with age. This study aims to investigate effects of 2 levels of pioglitazone (PIO) supplementation on peroxisome proliferator-activated receptor gamma (PPAR-γ) expression, semen quality, and fertility parameters of aged broiler breeder roosters. The efficacy of PIO was divided into 2 sections: receptor-dependent and receptor-independent. Expression of PPAR-γ mRNA and protein was assessed in sperm to monitor receptor-dependent actions. Sperm motility, velocity parameters, viability, mitochondrial activity, and apoptosis were assessed for the receptor-independent actions. Broiler breeder roosters were randomly assigned to 3 groups: 1) control received a basal diet (CTRL); 2) PIO-5 received a basal diet supplemented with 5 mg PIO/bird/day, and 3) PIO-10 received a basal diet supplemented with 10 mg PIO/bird/day. In addition, semen samples were collected from 24 Ross broiler breeder roosters at 30, 43, and 53 wk of age. Effects of PIO were significant in terms of total motility, straight-line velocity, mitochondrial activity, and apoptosis (P ≤ 0.05). Total motility, straight-line velocity and mitochondrial activity improved in both PIO groups (P ≤ 0.05) along with a significant reduction in early and late apoptosis in the PIO groups (P ≤ 0.05). Pioglitazone addition affected total motility, mitochondrial activity, early apoptosis and late apoptosis in a linearly and quadratically manner (P < 0.05). PPAR-γ mRNA and protein expression were not significantly upregulated by the different doses of PIO (P > 0.05). Similarly, fertility performance was not significantly changed in the PIO groups (P > 0.05). Moreover, PIO improved mitochondrial activity and decreased the apoptosis rate in the sperm of aged broiler breeder roosters. These improvements were associated with the receptor-independent actions of PIO and the mechanism of action of PIO did not appear to be affected by the PPAR-γ receptor in broiler breeder roosters.
公鸡的生育能力是肉种鸡繁殖中的一个重要课题，人们认为它与年龄有关。本研究旨在探讨 2 个水平的吡格列酮（PIO）补充对过氧化物酶体增殖物激活受体 γ（PPAR-γ）表达、精液质量和老龄肉种公鸡繁殖性能的影响。PIO 的功效分为 2 部分：受体依赖性和受体非依赖性。通过检测精子中 PPAR-γ mRNA 和蛋白的表达来监测受体依赖性作用。通过检测精子的运动性、速度参数、活力、线粒体活性和凋亡来评估受体非依赖性作用。将肉种公鸡随机分为 3 组：1）对照组接受基础日粮（CTRL）；2）PIO-5 组接受基础日粮，每天每只鸡补充 5mg PIO；3）PIO-10 组接受基础日粮，每天每只鸡补充 10mg PIO。此外，从 24 只罗斯肉种公鸡中收集精液样本，分别在 30、43 和 53 周龄时进行。结果表明，PIO 的影响在总活力、直线速度、线粒体活性和凋亡方面具有显著性（P≤0.05）。在 PIO 组中，总活力、直线速度和线粒体活性均得到改善（P≤0.05），同时 PIO 组的早期和晚期凋亡明显减少（P≤0.05）。PIO 的添加方式对总活力、线粒体活性、早期凋亡和晚期凋亡呈线性和二次方影响（P<0.05）。不同剂量的 PIO 对 PPAR-γ mRNA 和蛋白表达没有显著上调（P>0.05）。同样，在 PIO 组中，繁殖性能没有显著变化（P>0.05）。此外，PIO 改善了老龄肉种公鸡精子的线粒体活性并降低了凋亡率。这些改善与 PIO 的受体非依赖性作用有关，而 PIO 在肉种公鸡中的作用机制似乎不受 PPAR-γ 受体的影响。
