Xie Yan, Sutrisno Linawati, Yoshitomi Toru, Kawazoe Naoki, Yang Yingnan, Chen Guoping
Research Center for Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
Biomed Mater. 2022 Apr 13;17(3). doi: 10.1088/1748-605X/ac61f9.
Interconnected scaffolds are useful for promoting the chondrogenic differentiation of stem cells. Collagen scaffolds with interconnected pore structures were fabricated with poly(lactic acid--glycolic acid) (PLGA) sponge templates. The PLGA-templated collagen scaffolds were used to culture human bone marrow-derived mesenchymal stem cells (hMSCs) to investigate their promotive effect on the chondrogenic differentiation of hMSCs. The cells adhered to the scaffolds with a homogeneous distribution and proliferated with culture time. The expression of chondrogenesis-related genes was upregulated, and abundant cartilaginous matrices were detected. After subcutaneous implantation, the PLGA-templated collagen scaffolds further enhanced the production of cartilaginous matrices and the mechanical properties of the implants. The good interconnectivity of the PLGA-templated collagen scaffolds promoted chondrogenic differentiation. In particular, the collagen scaffolds prepared with large pore-bearing PLGA sponge templates showed the highest promotive effect.
相互连接的支架对于促进干细胞的软骨形成分化很有用。利用聚乳酸-乙醇酸共聚物（PLGA）海绵模板制备了具有相互连接孔隙结构的胶原支架。使用PLGA模板化的胶原支架培养人骨髓间充质干细胞（hMSCs），以研究其对hMSCs软骨形成分化的促进作用。细胞以均匀分布附着在支架上，并随着培养时间而增殖。软骨形成相关基因的表达上调，并且检测到大量软骨基质。皮下植入后，PLGA模板化的胶原支架进一步增强了软骨基质的产生以及植入物的力学性能。PLGA模板化胶原支架良好的相互连接性促进了软骨形成分化。特别是，用带有大孔隙的PLGA海绵模板制备的胶原支架显示出最高的促进效果。
