Park Kwideok, Cho Kyoung-Jin, Kim Jae-Jin, Kim Ik-Hwan, Han Dong Keun
Biomaterials Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Korea.
Macromol Biosci. 2009 Mar 10;9(3):221-9. doi: 10.1002/mabi.200800187.
Two chondrogenic factors, Dex and TGF-beta1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-beta1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage-specific genes and clearly displayed type-II collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF-beta1.
将两种软骨生成因子地塞米松（Dex）和转化生长因子-β1（TGF-β1）融入聚乳酸-羟基乙酸共聚物（PLGA）支架中，并评估其软骨生成潜力。将负载Dex的PLGA支架与丙烯酸（AA）和肝素进行接枝，然后使固定有肝素的支架与TGF-β1反应，得到PLGA/Dex-TGF（PLGA/D/T）支架。将兔间充质干细胞（MSCs）接种到支架上并培养4周。结果表明，包含软骨生成因子的支架强烈上调了软骨特异性基因的表达，并清晰显示出II型胶原蛋白免疫荧光。功能化的PLGA支架可为MSCs的软骨生成分化提供合适的微环境，而无需持续供应含Dex和TGF-β1的培养基。
