Isaksson L A, Takata R
Mol Gen Genet. 1978 Apr 25;161(1):9-14. doi: 10.1007/BF00266609.
The spontaneous temperature sensitive mutant 72c is shown to be more tolerant to fusidic acid, but less tolerant to trimethoprim on plates at permissive temperature, than is the parental strain. The poor growth of the mutant on amino acids supplemented plates, as well as its inability to grow on broth plates at 40 degrees, can be compensates by sublethal amounts of chloroamphenicol. Also some mutations to Rif-R or Str-R improve growth of the mutant under certain conditions. Reversion and other genetic analysis strongly suggest, that the pleiotropic behaviour of the mutant is due to a single mutation in a gene, which is designated fusB and is closely cotransducible with lip at min 14 of the E. coli chromosome. The gene order is lip-fusB-supE.
自发温度敏感突变体72c在允许温度下的平板上，被证明比亲本菌株对夫西地酸更具耐受性，但对甲氧苄啶的耐受性更低。该突变体在补充氨基酸的平板上生长不良，以及在40摄氏度的肉汤平板上无法生长，可通过亚致死量的氯霉素得到补偿。此外，一些突变为利福平抗性（Rif-R）或链霉素抗性（Str-R）的突变在某些条件下可改善该突变体的生长。回复突变和其他遗传分析强烈表明，该突变体的多效性行为是由于一个基因中的单一突变所致，该基因被命名为fusB，与大肠杆菌染色体14分钟处的lip紧密共转导。基因顺序为lip-fusB-supE。
