Sanchez-Fernandez C, Bolatti E M, Culasso A C A, Chouhy D, Kowalewski M M, Stella E J, Schurr T G, Rinas M A, Liotta D J, Campos R H, Giri A A, Badano I
Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Arch Virol. 2022 May;167(5):1257-1268. doi: 10.1007/s00705-022-05420-y. Epub 2022 Mar 30.
In this study, we investigated the occurrence of papillomavirus (PV) infection in non-human primates (NHPs) in northeastern Argentina. We also explored their evolutionary history and evaluated the co-speciation hypothesis in the context of primate evolution.
We obtained DNA samples from 57 individuals belonging to wild and captive populations of Alouatta caraya, Sapajus nigritus, and Sapajus cay. We assessed PV infection by PCR amplification with the CUT primer system and sequencing of 337 bp (112 amino acids) of the L1 gene. The viral sequences were analyzed by phylogenetic and Bayesian coalescence methods to estimate the time to the most common recent ancestor (t) using BEAST, v1.4.8 software. We evaluated viral/host tree congruence with TreeMap v3.0.
We identified two novel putative PV sequences of the genus Gammapapillomavirus in Sapajus spp. and Alouatta caraya (SPV1 and AcPV1, respectively). The t of SPV1 was estimated to be 11,941,682 years before present (ybp), and that of AcPV1 was 46,638,071 ybp, both before the coalescence times of their hosts (6.4 million years ago [MYA] and 6.8 MYA, respectively). Based on the comparison of primate and viral phylogenies, we found that the PV tree was no more congruent with the host tree than a random tree would be (P > 0.05), thus allowing us to reject the model of virus-host coevolution.
This study presents the first evidence of PV infection in platyrrhine species from Argentina, expands the range of described hosts for these viruses, and suggests new scenarios for their origin and dispersal.
在本研究中，我们调查了阿根廷东北部非人灵长类动物（NHPs）中乳头瘤病毒（PV）感染的发生情况。我们还探究了它们的进化历史，并在灵长类动物进化的背景下评估了共同物种形成假说。
我们从属于野生和圈养种群的57只卡拉亚吼猴（Alouatta caraya）、黑帽卷尾猴（Sapajus nigritus）和卡伊卷尾猴（Sapajus cay）个体中获取了DNA样本。我们使用CUT引物系统通过PCR扩增和对L1基因337 bp（112个氨基酸）进行测序来评估PV感染。使用BEAST v1.4.8软件通过系统发育和贝叶斯合并方法分析病毒序列，以估计最近共同祖先的时间（t）。我们使用TreeMap v3.0评估病毒/宿主树的一致性。
我们在卷尾猴属物种和卡拉亚吼猴中分别鉴定出两个新型的γ乳头瘤病毒属推定PV序列（分别为SPV1和AcPV1）。估计SPV1的t为距今11,941,682年前（ybp），AcPV1的t为46,638,071 ybp，两者均早于其宿主的合并时间（分别为640万年前[MYA]和680万年前）。基于灵长类动物和病毒系统发育的比较，我们发现PV树与宿主树的一致性并不比随机树更高（P>0.05），因此我们拒绝了病毒-宿主共同进化模型。
本研究提供了阿根廷阔鼻猴物种中PV感染的首个证据，扩大了这些病毒已知宿主的范围，并提出了它们起源和传播的新情况。
