Grupo Virología Humana, Instituto de Biología Molecular y Celular de Rosario (CONICET), Suipacha 590, 2000, Rosario, Argentina.
Área Virología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina.
BMC Infect Dis. 2020 Apr 7;20(1):274. doi: 10.1186/s12879-020-4893-3.
Human papillomaviruses (HPVs) have been divided into mucosal and cutaneous types according to their primary epithelial tissue tropism. However, recent studies showed the presence of several cutaneous types in mucosal lesions and healthy mucosa from different anatomical sites.
Here, the HPV prevalence and type-specific distribution were assessed in a variety of mucosal samples from 435 individuals using a combination of two established broad-spectrum primer systems: Gamma-PV PCR and CUT PCR.
Overall HPV prevalence in anal canal swabs, cervical cancer biopsies, genital warts and oral swabs was 85, 47, 62 and 4%, respectively. In anal canal swabs, Alpha-PVs were most frequently found (59%), followed by Gamma- (37%) and Beta-PVs (4%). The prevalence and persistence of HPV infection in the anal canal of 226 individuals were further explored. Overall HPV, Gamma-PVs and multiple HPV infections were significantly higher in men vs. women (p = 0.034, p = 0.027 and p = 0.003, respectively); multiple HPV infections were more common in individuals ≤40 years (p = 0.05), and significantly higher prevalence of Gamma-PVs and multiple HPV infections was observed in HIV-1-positive vs. HIV-1-negative individuals (p = 0.003 and p = 0.04, respectively). Out of 21 patients with follow-up anal swabs, only one persistent infection with the same type (HPV58) was detected.
Our findings suggest that Gamma-PVs (except species Gamma-6) are ubiquitous viruses with dual muco-cutaneous tissue tropism. Anal canal Gamma-PV infections may be associated with sexual behavior and the host immune status. This study expands the knowledge on Gamma-PVs' tissue tropism, providing valuable data on the characteristics of HPV infection in the anal canal.
根据其主要上皮组织嗜性，人乳头瘤病毒 (HPV) 已分为黏膜型和皮肤型。然而，最近的研究表明，几种皮肤型存在于不同解剖部位的黏膜病变和健康黏膜中。
在这里，使用两种已建立的广谱引物系统：Gamma-PV PCR 和 CUT PCR，评估了来自 435 个人的各种黏膜样本中的 HPV 流行情况和特定类型分布。
肛门拭子、宫颈癌活检、生殖器疣和口腔拭子中的 HPV 总流行率分别为 85%、47%、62%和 4%。在肛门拭子中，最常发现的是 Alpha-PVs（59%），其次是 Gamma-（37%）和 Beta-PVs（4%）。进一步探讨了 226 个人肛门 HPV 感染的流行情况和持续情况。总体 HPV、Gamma-PVs 和多重 HPV 感染在男性中明显高于女性（p=0.034、p=0.027 和 p=0.003）；多重 HPV 感染在≤40 岁的个体中更为常见（p=0.05），并且 HIV-1 阳性个体中 Gamma-PVs 和多重 HPV 感染的发生率明显更高（p=0.003 和 p=0.04）。在 21 名有随访肛门拭子的患者中，仅检测到一种相同类型（HPV58）的持续性感染。
我们的研究结果表明，Gamma-PVs（除了 Gamma-6 种）是具有双重黏膜皮肤组织嗜性的普遍存在的病毒。肛门 Gamma-PV 感染可能与性行为和宿主免疫状态有关。本研究扩展了对 Gamma-PVs 组织嗜性的认识，为肛门内 HPV 感染的特征提供了有价值的数据。
