Department of Paediatric Dentistry, Faculty of Dental Medicine, University of Rijeka, Kresimirova 42, 51000, Rijeka, Croatia.
Department of Orthodontics, Faculty of Dental Medicine, University of Rijeka, Kresimirova 42, 51000, Rijeka, Croatia.
Folia Microbiol (Praha). 2022 Aug;67(4):649-657. doi: 10.1007/s12223-022-00960-x. Epub 2022 Mar 30.
To explore the interaction effects of ciprofloxacin and ampicillin with nickel on the growth of bacteria, Staphylococcus aureus strain ATCC 29213, Enterococcus faecalis ATCC 29212 and Escherichia coli ATCC 25922 were used. Minimum inhibitory concentrations (MICs) were determined for nickel, ciprofloxacin and ampicillin, and the checkerboard method was used to assess their cumulative effects on bacterial growth. The interactions between the metal and antibiotics were assessed by the fractional inhibitory concentration (FIC). The MICs for ciprofloxacin and ampicillin were 0.31 and 1 mg/L for E. faecalis, 0.62 and 1 mg/L for S. aureus and 0.005 and 2.5 for E. coli, respectively. The MIC for nickel was 1000 mg/L for all bacteria. The FIC results for ciprofloxacin and nickel demonstrated an antagonistic effect of the two agents on the growth of E. coli and E. faecalis and an additive effect on S. aureus. The FICs for ampicillin and nickel demonstrated a synergistic effect on the growth of E. faecalis and E. coli. Different interactions of metals and antibiotics were observed depending on the bacteria and the type of antibiotic.
为了探索环丙沙星和氨苄西林与镍对细菌生长的相互作用效应，选用金黄色葡萄球菌（ATCC 29213）、粪肠球菌（ATCC 29212）和大肠杆菌（ATCC 25922）这 3 株细菌作为研究对象。测定镍、环丙沙星和氨苄西林对这 3 株细菌的最低抑菌浓度（MIC），并采用棋盘微量稀释法评估它们对细菌生长的累积效应。采用部分抑菌浓度指数（FIC）评估金属与抗生素之间的相互作用。粪肠球菌、金黄色葡萄球菌和大肠杆菌对环丙沙星和氨苄西林的 MIC 分别为 0.31 和 1 mg/L、0.62 和 1 mg/L、0.005 和 2.5 mg/L；镍对这 3 株细菌的 MIC 均为 1000 mg/L。环丙沙星和镍对大肠杆菌和粪肠球菌生长的 FIC 结果显示两者之间存在拮抗作用，对金黄色葡萄球菌的生长则表现为相加作用。氨苄西林和镍对粪肠球菌和大肠杆菌的生长表现为协同作用。不同的金属和抗生素对不同细菌的相互作用不同。
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