Soltani Rasool, Fazeli Hossein, Bahri Najafi Rahim, Jelokhanian Alireza
Department of Clinical Pharmacy and Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical sciences, Isfahan, Iran.
Department of Microbiology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran.
Iran J Pharm Res. 2017 Winter;16(1):290-296.
Antibiotic resistance is an important problem in antibiotic treatment of infections, particularly in hospitals. Tomatidine is a plant secondary metabolite with antimicrobial and antifungal effects. This study examined the possible synergistic effect tomatidine with several antibiotics against standard and clinical strains of , , and . After determining the minimum inhibitory concentrations (MICs) of antibiotics and tomatidine against the bacterial isolates using broth microdilution method, the synergistic effect between tomatidine and antibiotics was evaluated by checkerboard method and calculation of FIC indices. Tomatidine alone did not show any antimicrobial effect. However, it had synergistic effect with gentamicin and cefepime against standard and clinical isolates of and , respectively. It also had synergistic effect with ampicillin and ciprofloxacin only against standard strains of and , respectively. In conclusion, tomatidine could be considered as a potential antibiotic potentiator for gentamicin, cefepime and ciprofloxacin, and ampicillin against , , and infections, respectively. However, the toxicological and pharmacological properties of tomatidine for use as a therapeutic agent remain to be determined.
抗生素耐药性是感染性疾病抗生素治疗中的一个重要问题，在医院中尤为如此。番茄碱是一种具有抗菌和抗真菌作用的植物次生代谢产物。本研究考察了番茄碱与几种抗生素联合对标准菌株及临床分离的[具体细菌名称]的可能协同效应。采用肉汤微量稀释法确定抗生素和番茄碱对细菌分离株的最低抑菌浓度（MIC）后，通过棋盘法和FIC指数计算评估番茄碱与抗生素之间的协同效应。单独使用番茄碱未显示出任何抗菌作用。然而，它分别与庆大霉素和头孢吡肟对标准菌株及临床分离的[具体细菌名称]有协同效应。它还分别仅与氨苄西林和环丙沙星对标准菌株的[具体细菌名称]有协同效应。总之，番茄碱可分别被视为庆大霉素、头孢吡肟、环丙沙星和氨苄西林针对[具体细菌名称]感染的潜在抗生素增效剂。然而，番茄碱作为治疗剂的毒理学和药理学特性仍有待确定。
