Centre for Proteome Research, Institute of Systems and Integrative Biology, University of Liverpool, Liverpool, United Kingdom.
Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.
BMC Vet Res. 2022 Mar 30;18(1):121. doi: 10.1186/s12917-022-03202-x.
Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, progressive, interstitial fibrosing lung disease, manifesting as cough, exercise intolerance and ultimately, dyspnea and respiratory failure. It mainly affects West Highland white terriers (WHWTs), lacks curable treatment and has a poor prognosis. Aspiration of gastroesophageal refluxate may play a role in the development of CIPF. In the first part of this study, we completed label-free quantitative proteomic analysis of bronchoalveolar lavage fluid (BALF) from CIPF and healthy WHWTs. In the second part, we evaluated potential protein markers of reflux aspiration from canine gastric juice and vomitus and whether these were present in BALF from the two groups.
Across all BALF samples, 417 proteins were identified, and of these, 265 proteins were identified by two or more unique tryptic peptides. Using the 265 high confidence assignments, the quantitative proteome profiles were very similar in the two cohorts, but they could be readily resolved by principal component analysis on the basis of differential protein expression. Of the proteins that were differentially abundant in the two groups, several (including inflammatory and fibrotic markers) were elevated in CIPF, and a smaller, more diverse group of proteins were diminished in CIPF. No protein markers indicative of reflux aspiration were identified.
Label-free proteomics allowed discrimination between CIPF and healthy WHWTs, consistent with fibrotic process but did not provide clear evidence for gastrointestinal aspiration. The measurement of proteins may provide a proteomics signature of CIPF that could be used to evaluate treatment options.
犬特发性肺纤维化（CIPF）是一种慢性、进行性、间质纤维化性肺部疾病，表现为咳嗽、运动不耐受，最终出现呼吸困难和呼吸衰竭。它主要影响西部高地白梗犬（WHWT），缺乏可治愈的治疗方法，预后不良。胃食管反流物的吸入可能在 CIPF 的发展中起作用。在本研究的第一部分，我们完成了 CIPF 和健康 WHWT 支气管肺泡灌洗液（BALF）的无标记定量蛋白质组学分析。在第二部分，我们评估了来自犬胃液和呕吐物的潜在反流吸入蛋白标志物，以及这些标志物是否存在于两组 BALF 中。
在所有 BALF 样本中，鉴定出 417 种蛋白质，其中 265 种蛋白质被两个或更多独特的胰蛋白酶肽鉴定。使用 265 种高置信度分配，两组的定量蛋白质组谱非常相似，但可以根据差异表达的蛋白质通过主成分分析很容易区分。在两组中差异丰度的蛋白质中，一些（包括炎症和纤维化标志物）在 CIPF 中升高，而 CIPF 中减少的蛋白质数量较少，种类更多。没有发现提示反流吸入的蛋白质标志物。
无标记蛋白质组学允许区分 CIPF 和健康 WHWT，与纤维化过程一致，但没有提供明确的胃肠道吸入证据。蛋白质的测量可能为 CIPF 提供蛋白质组学特征，可用于评估治疗方案。
