富含β-石竹烯和氧化β-石竹烯的精油的抗伤害感受、抗炎和细胞毒性特性。

Antinociceptive, anti-inflammatory, and cytotoxic properties of essential oil, rich with β-caryophyllene and β-caryophyllene oxide.

作者信息

Moghrovyan Armenuhi, Parseghyan Lilya, Sevoyan Gohar, Darbinyan Anna, Sahakyan Naira, Gaboyan Monica, Karabekian Zaruhi, Voskanyan Armen

机构信息

Department of Pharmacognosy, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.

Orbeli Institute of Physiology, Laboratory of Physiologically Active Substances Investigations, Yerevan, Armenia.

出版信息

Korean J Pain. 2022 Apr 1;35(2):140-151. doi: 10.3344/kjp.2022.35.2.140.

BACKGROUND

Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflammatory effects in mice (in vivo) and cytotoxic action in cultured cells (in vitro). OVA essential oil was extracted from fresh plant material by hydro-distillation.

METHODS

For OVA essential oil contents determination the gas chromatography-mass spectrometry method was used. Formalin and hot plate tests and analysis of cell viability using the methyl-thiazolyl-tetrazolium (MTT) assay were used.

RESULTS

The maximal content of β-caryophyllene and β-caryophyllene oxide in OVA essential oil was revealed in the period of blossoming (8.18% and 13.36%, correspondently). In the formalin test, 4% OVA essential oil solution (3.5 mg/mouse) exerts significant antinociceptive and anti-inflammatory effects (P = 0.003). MTT assay shows approximately 60% cytotoxicity in HeLa and Vero cells for 2.0 μL/mL OVA essential oil in media.

CONCLUSIONS

The wild oregano herb of Armenian highlands, harvested in the blossoming period, may be considered as a valuable source for developing pain-relieving preparations.

背景

香精油因其镇痛和抗炎特性而备受关注。我们旨在研究亚美尼亚高地牛至（OVA）在不同植被时期的香精油含量，并研究其在小鼠体内的抗伤害感受和抗炎作用以及在培养细胞中的细胞毒性作用（体外）。OVA香精油通过水蒸馏法从新鲜植物材料中提取。

方法

采用气相色谱 - 质谱法测定OVA香精油含量。使用福尔马林和热板试验以及采用甲基噻唑基四氮唑（MTT）法分析细胞活力。

结果

OVA香精油中β - 石竹烯和氧化石竹烯的最大含量在开花期被检测到（分别为8.18%和13.36%）。在福尔马林试验中，4%的OVA香精油溶液（3.5毫克/小鼠）具有显著的抗伤害感受和抗炎作用（P = 0.003）。MTT分析表明，对于培养基中2.0微升/毫升的OVA香精油，HeLa和Vero细胞的细胞毒性约为60%。