• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

富含β-石竹烯和氧化β-石竹烯的精油的抗伤害感受、抗炎和细胞毒性特性。

Antinociceptive, anti-inflammatory, and cytotoxic properties of essential oil, rich with β-caryophyllene and β-caryophyllene oxide.

作者信息

Moghrovyan Armenuhi, Parseghyan Lilya, Sevoyan Gohar, Darbinyan Anna, Sahakyan Naira, Gaboyan Monica, Karabekian Zaruhi, Voskanyan Armen

机构信息

Department of Pharmacognosy, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.

Orbeli Institute of Physiology, Laboratory of Physiologically Active Substances Investigations, Yerevan, Armenia.

出版信息

Korean J Pain. 2022 Apr 1;35(2):140-151. doi: 10.3344/kjp.2022.35.2.140.

DOI:10.3344/kjp.2022.35.2.140
PMID:35354677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8977206/
Abstract

BACKGROUND

Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflammatory effects in mice (in vivo) and cytotoxic action in cultured cells (in vitro). OVA essential oil was extracted from fresh plant material by hydro-distillation.

METHODS

For OVA essential oil contents determination the gas chromatography-mass spectrometry method was used. Formalin and hot plate tests and analysis of cell viability using the methyl-thiazolyl-tetrazolium (MTT) assay were used.

RESULTS

The maximal content of β-caryophyllene and β-caryophyllene oxide in OVA essential oil was revealed in the period of blossoming (8.18% and 13.36%, correspondently). In the formalin test, 4% OVA essential oil solution (3.5 mg/mouse) exerts significant antinociceptive and anti-inflammatory effects (P = 0.003). MTT assay shows approximately 60% cytotoxicity in HeLa and Vero cells for 2.0 μL/mL OVA essential oil in media.

CONCLUSIONS

The wild oregano herb of Armenian highlands, harvested in the blossoming period, may be considered as a valuable source for developing pain-relieving preparations.

摘要

背景

香精油因其镇痛和抗炎特性而备受关注。我们旨在研究亚美尼亚高地牛至（OVA）在不同植被时期的香精油含量，并研究其在小鼠体内的抗伤害感受和抗炎作用以及在培养细胞中的细胞毒性作用（体外）。OVA香精油通过水蒸馏法从新鲜植物材料中提取。

方法

采用气相色谱 - 质谱法测定OVA香精油含量。使用福尔马林和热板试验以及采用甲基噻唑基四氮唑（MTT）法分析细胞活力。

结果

OVA香精油中β - 石竹烯和氧化石竹烯的最大含量在开花期被检测到（分别为8.18%和13.36%）。在福尔马林试验中，4%的OVA香精油溶液（3.5毫克/小鼠）具有显著的抗伤害感受和抗炎作用（P = 0.003）。MTT分析表明，对于培养基中2.0微升/毫升的OVA香精油，HeLa和Vero细胞的细胞毒性约为60%。

结论

在开花期收获的亚美尼亚高地野生牛至草可被视为开发止痛制剂的宝贵来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/1fd9860a7214/kjp-35-2-140-f13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/2128bc4d94ac/kjp-35-2-140-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/1b7000f93df8/kjp-35-2-140-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/9b88e545bb83/kjp-35-2-140-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/d55b787238c1/kjp-35-2-140-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/4866c6e38e90/kjp-35-2-140-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/2292865ad47c/kjp-35-2-140-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/9ef242487cc7/kjp-35-2-140-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/4bd66858655b/kjp-35-2-140-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/09cda92fcf2e/kjp-35-2-140-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/2f4ce39495bb/kjp-35-2-140-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/5419282fc93b/kjp-35-2-140-f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/d2cdd6d16ab0/kjp-35-2-140-f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/1fd9860a7214/kjp-35-2-140-f13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/2128bc4d94ac/kjp-35-2-140-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/1b7000f93df8/kjp-35-2-140-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/9b88e545bb83/kjp-35-2-140-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/d55b787238c1/kjp-35-2-140-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/4866c6e38e90/kjp-35-2-140-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/2292865ad47c/kjp-35-2-140-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/9ef242487cc7/kjp-35-2-140-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/4bd66858655b/kjp-35-2-140-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/09cda92fcf2e/kjp-35-2-140-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/2f4ce39495bb/kjp-35-2-140-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/5419282fc93b/kjp-35-2-140-f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/d2cdd6d16ab0/kjp-35-2-140-f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f824/8977206/1fd9860a7214/kjp-35-2-140-f13.jpg

相似文献

1
Antinociceptive, anti-inflammatory, and cytotoxic properties of essential oil, rich with β-caryophyllene and β-caryophyllene oxide.富含β-石竹烯和氧化β-石竹烯的精油的抗伤害感受、抗炎和细胞毒性特性。
Korean J Pain. 2022 Apr 1;35(2):140-151. doi: 10.3344/kjp.2022.35.2.140.
2
Effect of prolonged water stress on essential oil content, compositions and gene expression patterns of mono- and sesquiterpene synthesis in two oregano (Origanum vulgare L.) subspecies.长期水分胁迫对两种牛至（牛至属植物）亚种中精油含量、成分以及单萜和倍半萜合成基因表达模式的影响
Plant Physiol Biochem. 2017 Feb;111:119-128. doi: 10.1016/j.plaphy.2016.11.023. Epub 2016 Nov 28.
3
Essential Oil and Ethanol Extract of Oregano (Origanum vulgare L.) from Armenian Flora as a Natural Source of Terpenes, Flavonoids and other Phytochemicals with Antiradical, Antioxidant, Metal Chelating, Tyrosinase Inhibitory and Antibacterial Activity.从亚美尼亚植物群中提取的牛至（Oregano，Origanum vulgare L.）精油和乙醇提取物作为具有自由基清除、抗氧化、金属螯合、酪氨酸酶抑制和抗菌活性的萜类化合物、类黄酮和其他植物化学物质的天然来源。
Curr Pharm Des. 2019;25(16):1809-1816. doi: 10.2174/1381612825666190702095612.
4
Role of β-Caryophyllene in the Antinociceptive and Anti-Inflammatory Effects of Cav. Essential Oil.β-石竹烯在 Cav.精油的镇痛和抗炎作用中的作用。
Molecules. 2020 Feb 5;25(3):675. doi: 10.3390/molecules25030675.
5
Chemical composition and evaluation of the anti-inflammatory and antinociceptive activities of Duguetia furfuracea essential oil: Effect on edema, leukocyte recruitment, tumor necrosis factor alpha production, iNOS expression, and adenosinergic and opioidergic systems.化学组成与分析杜茎山挥发油的抗炎与镇痛活性：对水肿、白细胞募集、肿瘤坏死因子-α生成、诱导型一氧化氮合酶表达及腺苷能和阿片能系统的影响。
J Ethnopharmacol. 2019 Mar 1;231:325-336. doi: 10.1016/j.jep.2018.11.017. Epub 2018 Nov 13.
6
The essential oil of Origanum vulgare L. ssp. vulgare growing wild in vilnius district (Lithuania).在维尔纽斯地区（立陶宛）野生生长的牛至（Origanum vulgare L. ssp. vulgare）的精油。
Phytochemistry. 2001 May;57(1):65-9. doi: 10.1016/s0031-9422(00)00474-x.
7
Anti-Inflammatory, Antioxidant, Antibiotic, and Cytotoxic Activities of Tanacetum vulgare L. Essential Oil and Its Constituents.艾菊精油及其成分的抗炎、抗氧化、抗菌和细胞毒性活性
Medicines (Basel). 2017 May 25;4(2):34. doi: 10.3390/medicines4020034.
8
Chemical composition and biological activity of the essential oil obtained from Bupleurum marginatum (Apiaceae).从竹叶柴胡（伞形科）中提取的挥发油的化学成分及生物活性
J Pharm Pharmacol. 2009 Aug;61(8):1079-87. doi: 10.1211/jpp/61.08.0012.
9
Essential oil diversity of European Origanum vulgare L. (Lamiaceae).欧洲牛至（唇形科）精油的多样性
Phytochemistry. 2015 Nov;119:32-40. doi: 10.1016/j.phytochem.2015.09.008. Epub 2015 Oct 8.
10
Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.在体内模型中，野桐（大戟科）精油和化合物 β-石竹烯的抗炎和抗水肿作用。
Phytomedicine. 2018 Mar 1;41:82-95. doi: 10.1016/j.phymed.2018.02.004. Epub 2018 Feb 13.

引用本文的文献

1
Optimized and Functionalized Carvacrol-Loaded Nanostructured Lipid Carriers for Enhanced Cytotoxicity in Breast Cancer Cells.用于增强乳腺癌细胞细胞毒性的优化且功能化的载香芹酚纳米结构脂质载体
Pharmaceutics. 2025 Mar 13;17(3):363. doi: 10.3390/pharmaceutics17030363.
2
The analgesic and anti-inflammatory effects of a combined preparation based on the blunt-nosed viper's venom and oregano essential oil.一种基于钝鼻蝰蛇毒液和牛至精油的复方制剂的镇痛和抗炎作用。
Korean J Pain. 2025 Apr 1;38(2):163-176. doi: 10.3344/kjp.24301. Epub 2025 Mar 26.
3
3D Printed Bigel: A Novel Delivery System for Cannabidiol-Rich Hemp Extract.

本文引用的文献

1
L. (Za'atar), from Raw to Go: A Review.L.（扎塔尔），从原料到成品：综述。
Plants (Basel). 2021 May 17;10(5):1001. doi: 10.3390/plants10051001.
2
Cannabinoid control of neurogenic inflammation.大麻素对神经源性炎症的调控。
Br J Pharmacol. 2020 Oct;177(19):4386-4399. doi: 10.1111/bph.15208. Epub 2020 Aug 13.
3
A Systematic Review of Essential Oils and the Endocannabinoid System: A Connection Worthy of Further Exploration.精油与内源性大麻素系统的系统评价：值得进一步探索的联系
3D打印软胶囊：一种用于富含大麻二酚的大麻提取物的新型给药系统。
Gels. 2024 Nov 26;10(12):770. doi: 10.3390/gels10120770.
4
Traditionally Used Medicinal Plants of Armenia.亚美尼亚的传统药用植物。
Plants (Basel). 2024 Dec 4;13(23):3411. doi: 10.3390/plants13233411.
5
Additive effect of clove essential oil combined with hydrogen inhalation improves psychological harm caused by lipopolysaccharide in mice.丁香精油联合氢气吸入对脂多糖引起的小鼠心理伤害具有协同作用。
BMC Complement Med Ther. 2024 Nov 15;24(1):399. doi: 10.1186/s12906-024-04682-0.
6
Synergistic Antinociceptive Effect of β-Caryophyllene Oxide in Combination with Paracetamol, and the Corresponding Gastroprotective Activity.氧化β-石竹烯与对乙酰氨基酚联合使用的协同镇痛作用及相应的胃保护活性。
Biomedicines. 2024 May 8;12(5):1037. doi: 10.3390/biomedicines12051037.
7
A L. Non-Polar Extract Modulates the Polarization of Human Macrophages and Dendritic Cells into an Anti-Inflammatory Phenotype.A L. 非极性提取物可将人巨噬细胞和树突状细胞的极化状态调节为抗炎表型。
Molecules. 2023 Oct 14;28(20):7094. doi: 10.3390/molecules28207094.
8
Mediterranean Plants with Antimicrobial Activity against , a Meta-Analysis for Green Veterinary Pharmacology Applications.具有抗……抗菌活性的地中海植物，绿色兽医药理学应用的荟萃分析
Microorganisms. 2023 Sep 9;11(9):2264. doi: 10.3390/microorganisms11092264.
9
Creation of New Oregano Genotypes with Different Terpene Chemotypes via Inter- and Intraspecific Hybridization.通过种间和种内杂交创建具有不同萜烯化学型的新型牛至基因型。
Int J Mol Sci. 2023 Apr 15;24(8):7320. doi: 10.3390/ijms24087320.
10
The Role of Oxidative Stress-Mediated Inflammation in the Development of T2DM-Induced Diabetic Nephropathy: Possible Preventive Action of Tannins and Other Oligomeric Polyphenols.氧化应激介导的炎症在 T2DM 诱导的糖尿病肾病发展中的作用：单宁和其他低聚多酚的可能预防作用。
Molecules. 2022 Dec 18;27(24):9035. doi: 10.3390/molecules27249035.
Evid Based Complement Alternat Med. 2020 May 15;2020:8035301. doi: 10.1155/2020/8035301. eCollection 2020.
4
The Cultivable Bacterial Microbiota Associated to the Medicinal Plant L.: From Antibiotic Resistance to Growth-Inhibitory Properties.与药用植物L.相关的可培养细菌微生物群：从抗生素抗性到生长抑制特性
Front Microbiol. 2020 May 8;11:862. doi: 10.3389/fmicb.2020.00862. eCollection 2020.
5
The Caucasian flora: a still-to-be-discovered rich source of antioxidants.高加索植物区系：一个有待发现的抗氧化剂丰富来源。
Free Radic Res. 2019;53(sup1):1153-1162. doi: 10.1080/10715762.2019.1648799. Epub 2019 Sep 12.
6
Essential Oil and Ethanol Extract of Oregano (Origanum vulgare L.) from Armenian Flora as a Natural Source of Terpenes, Flavonoids and other Phytochemicals with Antiradical, Antioxidant, Metal Chelating, Tyrosinase Inhibitory and Antibacterial Activity.从亚美尼亚植物群中提取的牛至（Oregano，Origanum vulgare L.）精油和乙醇提取物作为具有自由基清除、抗氧化、金属螯合、酪氨酸酶抑制和抗菌活性的萜类化合物、类黄酮和其他植物化学物质的天然来源。
Curr Pharm Des. 2019;25(16):1809-1816. doi: 10.2174/1381612825666190702095612.
7
Analysis of Cell Viability by the MTT Assay.通过MTT法分析细胞活力。
Cold Spring Harb Protoc. 2018 Jun 1;2018(6):2018/6/pdb.prot095505. doi: 10.1101/pdb.prot095505.
8
Non-clinical toxicity of β-caryophyllene, a dietary cannabinoid: Absence of adverse effects in female Swiss mice.β-石竹烯的非临床毒性：雌性瑞士小鼠未出现不良反应。
Regul Toxicol Pharmacol. 2018 Feb;92:338-346. doi: 10.1016/j.yrtph.2017.12.013. Epub 2017 Dec 16.
9
Essential Oils of Oregano: Biological Activity beyond Their Antimicrobial Properties.牛至精油：超越抗菌特性的生物活性。
Molecules. 2017 Jun 14;22(6):989. doi: 10.3390/molecules22060989.
10
β-caryophyllene and β-caryophyllene oxide-natural compounds of anticancer and analgesic properties.β-石竹烯和氧化β-石竹烯——具有抗癌和止痛特性的天然化合物。
Cancer Med. 2016 Oct;5(10):3007-3017. doi: 10.1002/cam4.816. Epub 2016 Sep 30.