Scarsella Elisa, Meineri Giorgia, Sandri Misa, Ganz Holly H, Stefanon Bruno
AnimalBiome, 400 29th Street, Suite 101, Oakland, CA 94609, USA.
Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle Scienze 206, 33100 Udine, Italy.
Vet Sci. 2023 Apr 5;10(4):277. doi: 10.3390/vetsci10040277.
Recent studies have found bacterial DNA in the blood of healthy individuals. To date, most studies on the blood microbiome have focused on human health, but this topic is an expanding research area in animal health as well. This study aims to characterize the blood microbiome of both healthy dogs and those with chronic gastro-enteropathies. For this study, blood and fecal samples were collected from 18 healthy and 19 sick subjects, DNA was extracted through commercial kits, and the V3-V4 regions of the 16S rRNA gene were sequenced on the Illumina platform. The sequences were analyzed for taxonomic annotation and statistical analysis. Alpha and beta diversities of fecal microbiome were significantly different between the two groups of dogs. Principal coordinates analysis revealed that healthy and sick subjects were significantly clustered for both blood and fecal microbiome samples. Moreover, bacterial translocation from the gut to the bloodstream has been suggested because of found shared taxa. Further studies are needed to determine the origin of the blood microbiome and the bacteria viability. The characterization of a blood core microbiome in healthy dogs has potential for use as a diagnostic tool to monitor for the development of gastro-intestinal disease.
最近的研究在健康个体的血液中发现了细菌DNA。迄今为止，大多数关于血液微生物组的研究都集中在人类健康方面，但这个主题在动物健康领域也是一个不断扩展的研究领域。本研究旨在描述健康犬和患有慢性胃肠病的犬的血液微生物组特征。在本研究中，从18只健康犬和19只患病犬采集了血液和粪便样本，通过商业试剂盒提取DNA，并在Illumina平台上对16S rRNA基因的V3-V4区域进行测序。对序列进行分类注释和统计分析。两组犬粪便微生物组的α和β多样性存在显著差异。主坐标分析表明，健康犬和患病犬的血液和粪便微生物组样本均明显聚类。此外，由于发现了共享分类群，提示存在细菌从肠道向血液的易位。需要进一步研究以确定血液微生物组的来源和细菌活力。健康犬血液核心微生物组的特征有潜力用作监测胃肠疾病发展的诊断工具。
