Department of Pharmacology, Babol University of Medical Science, Babol, Iran.
Department of Environmental Sciences, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares University, Noor, Iran.
J Environ Public Health. 2022 Mar 21;2022:4593835. doi: 10.1155/2022/4593835. eCollection 2022.
Crystals of malachite green (MG), being water-soluble, are effective agents applied to combat fungal and parasitic infections in fish. This study was conducted to evaluate the adsorption of MG as a cationic dye by polymeric resin Amberlyst 15. Changes in several parameters were observed, including the concentration of MG, pH, the adsorption rate and extent, and the temperature that might all affect the efficiency of adsorption. The adsorption method was described well by both the Redlich-Peterson and Langmuir isotherms with of 0.9933 and 0.9880, respectively. The kinetic information is consistent with the Freundlich isotherm model and pseudo-2nd-order kinetics model. Analysis of malachite green was executed by HPLC containing a Eurospher 100-5 C18 (25 cm × 4.5 mm, size of particle 5 m) column, UV detector was set at 618 nm, and 125 mM ammonium acetate was adapted to pH 4.5 with formic acid-acetonitrile (45 : 55, v/v) as the moving phase. The limit of the discovery factor was 0.02 gL. The negative value of Δ° reveals the spontaneity of the absorption method. The positive value of Δ° (333.1253 J/K mol) gives back randomness at the solid-liquid interface of sorption. The required adsorbent concentration was calculated for removing MG up to an extraction efficiency of 98.27% after 240 minutes.
孔雀石绿（MG）是一种水溶性晶体，作为一种阳离子染料，常用于对抗鱼类的真菌和寄生虫感染。本研究旨在评估聚合树脂 Amberlyst 15 对 MG 的吸附作用。我们观察了多种参数的变化，包括 MG 的浓度、pH 值、吸附速率和程度，以及可能影响吸附效率的温度。Redlich-Peterson 和 Langmuir 等温线都很好地描述了吸附方法，分别为 0.9933 和 0.9880。动力学信息与 Freundlich 等温线模型和拟二级动力学模型一致。含有 Eurospher 100-5 C18（25 cm×4.5 mm，粒径 5 μm）柱的 HPLC 用于分析孔雀石绿，UV 检测器设置在 618 nm，125 mM 乙酸铵适应 pH 4.5，使用甲酸-乙腈（45：55，v/v）作为流动相。发现因子的限值为 0.02 gL。Δ°的负值表明吸收方法的自发性。Δ°（333.1253 J/K mol）的正值反映了吸附固液界面的随机性。计算出所需的吸附剂浓度，以在 240 分钟后达到 98.27%的萃取效率去除 MG。
